What are the possible reasons for consistent decline in the cashmere production?

Cashmere production and productivity.

12 November 2020 2,106 2 View

While inserting GOI in pCW57.1 lentiviral plasmid DNA, is it important to delete ccdB gene fragment?

I am facing a problem with the plasmid from addgene (pcW57.1), when transformed into chemically competent DHFa cells, did not give any single colony overnight in ampicilin containing agar...

17 July 2020 8,001 1 View

Changing Aspen Plus non-conventional component into a mixture of few conventional compounds?

I have performed a bio-refinery simulation on Aspen Plus using non-conventional components. Now, what I am willing to do is to specify one of the product (non-conventional) into a mixture of some...

12 February 2020 5,469 4 View

What is the best journal to publish a review article regarding different fasting methods and effect of fasting on human health.?

Abstract Fasting is an important commitment of Islam and one of the fundamentals of Muslim life. By abstinence from all types of foods from dawn to dusk typically can provide great health...

24 September 2018 2,960 2 View

I do ms-PCR of fmr1 gene (GC content above 90%) after bisulfite treatment by using Zymo Gold kit. But I do not get amplification. Why?

A number of labs. have already done it successfully. Also I am using an already designed primer pair, following the same PCR profile and programme.

23 July 2018 9,366 3 View

How analysis of variance for augmented design can be done conveniently??

22 April 2016 6,287 3 View

Why is the user subroutine terminating the analysis?

i am getting an error ***ERROR: Analysis is being terminated from a user subroutine. Apparently i cannot pinpoint the exact source of the error. I am not sure but i can just guess that its due to...

25 February 2016 2,220 1 View

Can we find refractive index by using Kubelka-Munk Function [F(R)]?

can we find refractive index by using Kubelka-Munk Function [F(R)]??

26 December 2015 4,737 2 View

What is the ideal gene for internal control in real-time PCR? Is is 18SRNA or G6PD?

Hi, What is the ideal gene for internal control in real-time PCR? 18SRNA or G6PD? I am working on the Leukemia(AML) patient samples(bone marrow/blood..RNA extraction), and when I do PCR on cDNA...

19 January 2015 2,198 7 View

Dear's, Is it possible to separate the Lysine proteins through electrophoresis?

01 January 1970 712 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View