Sepideh Moori

6 Questions 5 Answers 0 Followers

Questions related from Sepideh Moori

I have question about transduction with pCDH-CMV-MCS-EF1-CopGFP-T2A-Puro vector?

Greetings, recently I have acquired the pCDH-CMV-MCS-EF1-CopGFP-T2A-Puro vector (V012884). I would like to know whether the gene of interest's expression is regulated by the CMV promoter or not,...

23 May 2024 8,085 2 View

Is it possible to run a PCR with these primers?

Hello, I want to use a multiple(2) fragments In-fusion cloning technique, so I tried to design four primers with snapgene and takara online tool, but all of the primers have a Tm more than 65 °C...

15 December 2022 6,513 4 View

Do all cell's surface proteins exist on the exosome surface too?

Is there any protein that expresses on parental cell surface but dose not exist on its exosome's surface?

22 November 2022 4,621 3 View

Where to add signal peptide in the protein?

I wanted to add a signal peptide to the extracellular domain of the protein so that it will be localized on the membrane. how to know which signal peptide to add and where to add it (N_ terminal...

21 October 2022 1,460 1 View

Hello, I have plasmid extraction problem, what is going wrong in your opinion?

Hello, I have transformed pcDNA vector into DH5 alpha bacteria and confirmed it with antibiotic test, then I did PCR colony and I also observed the band related to my gene, but after the plasmid...

22 September 2022 5,765 4 View

Can only the spike protein of the virus provide immunity?

Virology, microbiology, immunology, vaccine

22 December 2020 603 16 View