Hi,

i am working on effects of gemcitabine in HepG2 cell line and i should use the IC50 of Gemcitabine in the experiments.i found different values in literature and the numbers are highly variable ranging from lower nanomolar to lower micromolar for the same cell line under same conditions.!!!!!!

but in my tests,which i use different dose of gemcitabine from 0.25 to 16 micro molar ,from dose up to 1 micro molar ,i got same viability percentage.(60 percent)for all of drug doses.and also when i look at my cells,their morphology change a little bit and cells become wider like they are in G1 phase of cell cycle and the apoptotic cells are not exist in my plates.i think that the drug arrest my cells proliferation.i cant get the IC50.

is it normal ???

what should i do?

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