I have interest in Protein/ Enzyme engineering and wanted some hands-on. Can anyone suggest how and where to start?

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Need a reference on pig residual analysis report and the parameters containing in it, for an oil and gas pipeline industry. Has anybody done it?

Pigging is basically a cleansing process followed in all pipeline industries after the product has been delivered. There are many types of pigs that are inserted inside the pipeline for this...

27 June 2024 1,221 1 View

HI, How to find phase response in CST?

I'm simulating a metamaterial unit cell. I want to plot Frequency Vs Phase Response. Also I want to extract permeability and permittivity. Kindly share the steps.

23 June 2024 3,446 1 View

Yeast Cell Glucose Uptake Assay ? Can yeast release more glucose into the reaction, when treated with test compound ?

Hi all, We performed Yeast-Cell-Glucose-Uptake assay to estimate the antidiabetic potential of the test compound. The Metformin treated yeast cells, resulted in taking up more glucse than normal...

02 April 2024 7,657 2 View

Suggest good geospatial data security papers ?

pursuing phd on geospatial data security.suggest good paper on robust invisible blind watermarking for copyright protection

12 March 2024 4,917 2 View

What is the difference between the single microgrid and multi microgrid? why we consider Multi microgrid in an area but not as a single microgrid ?

in a community area, there is an multi microgrid systems, why we call it as multi microgrid system but not treated as single microgrid system. and difference between single microgrid and multi...

03 March 2024 9,306 1 View

Wire bonding issue (Au/Ti/GaN). Any feasible solutions please?

I am facing an issue of wire bonding, trying to ball bond Au wire onto a metal stack/semiconductor (Au/Ti/GaN). Thickness of Au = 200 nm, Thickness of Ti = 20 nm. I observe that the metal...

07 January 2024 4,813 1 View

How to calculate the precision for a RT PCR assay?

I am studying precision for my assay/kit with the following parameters followed: Panel members to be tested: 1. Weak positive 2. Medium positive 3. Strong positive 4. Negative All these were...

11 December 2023 1,812 1 View

How to add my articles in research gate?

how can i add all my articles and journals in research gate including those published in research gate?

22 November 2023 5,926 3 View

Statins causes liver damage upon prolonged use. To control it, given natural with hepatoprotective activity. But results HDI! what might be the cause?

Atorvastatin used to treat Atherosclerosis, seems to causes hepatoxicity upon prolonged usage. So, to inhibit such problem we have introduced an natural substances which is reported for the...

09 November 2023 587 0 View

Why monocytes adhere while differentiating them as Dendritic cells?

I prepared PBMCs,s from human blood and differentiated them as immature dendritic cells in this process, monocytes adhere to the plates. What could be the reason for this phenotype?

09 November 2023 8,266 2 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How to get links for copyrights for papers?

how to get links for copyrights for papers?

06 August 2024 7,410 1 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

How do i get an account to upload my published papers?

need to open an account to upload my published papers

01 August 2024 9,255 1 View

Could someone explain the basics of working with enzymes?

I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...

01 August 2024 5,460 5 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

31 July 2024 7,259 1 View

Any tips on how to increase the chances of getting a response?

I've been using ResearchGate for a while now and have found many useful research papers that are beneficial for my work. However, I've noticed that many authors do not respond to my messages....

31 July 2024 7,771 5 View

Is the black sediment consider as sand? If not, how can I filter it out?

Hi researchers! I'm working on soil texture analysis, and the end result for sand is doubtful because there is black sediment appearing after drying, as shown in the figure. Is it considered sand?...

30 July 2024 557 2 View

What are some diseases that are caused by overactivity of enzymes?

I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...

30 July 2024 2,483 5 View

Mohammad Mizanur Rahman

Hi Ravi, I think you can read some articles related to protein expression and purification, also how to perform SDS PAGE gel electrophoresis.

Dominique Liger

Hi there,

Protein engineering is such a wide field of research that you should first decide what you want to do more precisely...

Ravi Jhunjhunwala

Dominique Liger I want to work precisely on enzyme/ protein stabilization by improving their properties.

Mª Angeles Zorrilla Lopez-Perea

I would like to read the rest of the answers when they come