This is common phenomenon in most cell differentiation protocol. You have to be very careful while changing the media as they loos binding from the substratum. You should change one well at the one time and then go for next. This is time consuming but worked for me.
If possible you can use coated plates, now a days they are commercially available but they are costly. Or you can coat by yourself with poly-l-lysin.
Good luck
Also, check the origin of your cells. Are they bona fide C2C12 from ATCC or other trusted sources?
I remember having problems with C2C12 detaching from the plates until I switched to a new batch from a different origin.
The main issue is when i seeds them with coverlips, after the 4th day of differentiation they start to peel off the glass. I´ve tried really carefully and slowly when it comes to the medium change but still occurs. Any idea why ?
Thanks again for the replies !!
Hi Matias
Which EM are you using? how is the differentiation state at 4th day?
Cheers
C
Hi Carlos, differentiation goes well,currently using for differentiation DMEM 2%HS. Any suggestion? Besides being careful with the medium change?
Thanks!
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