I want to know how i can be certain about formation of inclusion body during protein expression?

I want to express a recombinant form of protein in bacteria and i'm not sure about this protein expression is soluble form. I want to know is there any way to be certain that it will be expressed...

08 September 2019 9,112 10 View

How can monitor how much studies are done on special matter?

I want to know when rate of application special kind of treatment started and how much studies exctlly are done on this matter . Actually is there any website to show rate of publication about...

08 September 2018 8,206 3 View

Does the enzyme immobilized on nano carrier based on FTIR spectra?

I want to immobilize my enzyme on ZIF-8 with covalent and entrapment techniques.I use DCC (dicyclohexyle carbodiimide)for carrier activation. Does this FTIR spectra prove immobilization?

05 June 2018 261 0 View

How can one reduce As+3 to As0 without interference of As+5?

01 February 2015 3,881 3 View

Can a circular dichroism monitor immobilization effect on protein structure?

31 December 2014 9,525 6 View

Can anyone help me with FTIR interpretation?

I immobilize enzyme on chitosan macrobead with Glutaraldehyde as crosslinker. I want to know how FTIR confirm immobilization? Which wavelength is related to chitosan with glutaraldeyde and which...

31 December 2014 2,603 11 View

Why doesn't my protein have desirable activity?

I work on cellulase enzyme and express it in E.coli(BL21). In SDS-PAGE I have good bands, but after purification with Ni-NTA column and dialysis, it's activity is very low (according to enzymatic...

05 June 2014 6,083 16 View

What is my problem in cloning?

I want to clone my gene to expreesion vector in Ecoli, after I clean my pcr product, I have a good band. In digestion step, after digesting the gene I don't get any band, but I perform a ligation...

01 February 2014 5,012 17 View

Why is my PCR band is so weak?

I do PCR reaction for my gene (it's about1600bp). Nonspecific bands are sharper than my my specific band. I examined temperature gradient, different template concentration (template is vector...

11 December 2013 1,730 16 View

Can someone advise me why my protein fails to express?

I work on eukaryotic gene, I want to clone and express it, I transform my recombinant plasmid to Ecoli(BL21), after induction, my protein does not express. I don't see my protein band in SDS...

11 December 2013 4,682 7 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View