I want to know how i can be certain about formation of inclusion body during protein expression?

I want to express a recombinant form of protein in bacteria and i'm not sure about this protein expression is soluble form. I want to know is there any way to be certain that it will be expressed...

08 September 2019 9,112 10 View

Does the enzyme immobilized on nano carrier based on FTIR spectra?

I want to immobilize my enzyme on ZIF-8 with covalent and entrapment techniques.I use DCC (dicyclohexyle carbodiimide)for carrier activation. Does this FTIR spectra prove immobilization?

05 June 2018 261 0 View

How can one reduce As+3 to As0 without interference of As+5?

01 February 2015 3,881 3 View

Can a circular dichroism monitor immobilization effect on protein structure?

31 December 2014 9,525 6 View

Can anyone help me with FTIR interpretation?

I immobilize enzyme on chitosan macrobead with Glutaraldehyde as crosslinker. I want to know how FTIR confirm immobilization? Which wavelength is related to chitosan with glutaraldeyde and which...

31 December 2014 2,603 11 View

Why doesn't my protein have desirable activity?

I work on cellulase enzyme and express it in E.coli(BL21). In SDS-PAGE I have good bands, but after purification with Ni-NTA column and dialysis, it's activity is very low (according to enzymatic...

05 June 2014 6,083 16 View

What is my problem in cloning?

I want to clone my gene to expreesion vector in Ecoli, after I clean my pcr product, I have a good band. In digestion step, after digesting the gene I don't get any band, but I perform a ligation...

01 February 2014 5,012 17 View

I do gel cracking for separate bands, unfortunately I only see a smear in each well. Why only the weak gene band is visible?

I reculture each colony in another plate after transformation and use them for cracking in the cracking buffer (SDS,NaOH,EDTA,D2O), I run them with 10 micro dye hardly but I don't see plasmid...

31 December 2013 8,203 1 View

Why is my PCR band is so weak?

I do PCR reaction for my gene (it's about1600bp). Nonspecific bands are sharper than my my specific band. I examined temperature gradient, different template concentration (template is vector...

11 December 2013 1,730 16 View

Can someone advise me why my protein fails to express?

I work on eukaryotic gene, I want to clone and express it, I transform my recombinant plasmid to Ecoli(BL21), after induction, my protein does not express. I don't see my protein band in SDS...

11 December 2013 4,682 7 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Any suggestions for a website that allows you to create questionnaires and gather unlimited data (Totally free)?

I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you

28 February 2021 3,237 3 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

How to get rid of somebody pretending to be the author of a publication?

One of the publication I co-authored has the author's name in abbreviation. Then, somebody whose nothing to do with that paper acquired it as her work. To whom in this RG shall I ask for a hand,...

27 February 2021 4,206 4 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View