I want to immobilize my enzyme on ZIF-8 with covalent and entrapment techniques.I use DCC (dicyclohexyle carbodiimide)for carrier activation. Does this FTIR spectra prove immobilization?
I want to express a recombinant form of protein in bacteria and i'm not sure about this protein expression is soluble form. I want to know is there any way to be certain that it will be expressed...
08 September 2019 9,257 10 View
I want to know when rate of application special kind of treatment started and how much studies exctlly are done on this matter . Actually is there any website to show rate of publication about...
08 September 2018 8,341 3 View
When we have two forms of As in an environment, how and in which potential does the reduction of As+3 to As0 occur, and the interference of As+5 form is zero?
01 February 2015 4,032 3 View
When you immobilized enzyme on polymeric support can you detect changes in protein content (αhelix% or β sheet% in enzyme)?
31 December 2014 9,684 6 View
I immobilize enzyme on chitosan macrobead with Glutaraldehyde as crosslinker. I want to know how FTIR confirm immobilization? Which wavelength is related to chitosan with glutaraldeyde and which...
31 December 2014 2,745 11 View
I work on cellulase enzyme and express it in E.coli(BL21). In SDS-PAGE I have good bands, but after purification with Ni-NTA column and dialysis, it's activity is very low (according to enzymatic...
05 June 2014 6,230 16 View
I want to clone my gene to expreesion vector in Ecoli, after I clean my pcr product, I have a good band. In digestion step, after digesting the gene I don't get any band, but I perform a ligation...
01 February 2014 5,375 17 View
I reculture each colony in another plate after transformation and use them for cracking in the cracking buffer (SDS,NaOH,EDTA,D2O), I run them with 10 micro dye hardly but I don't see plasmid...
31 December 2013 8,350 1 View
I do PCR reaction for my gene (it's about1600bp). Nonspecific bands are sharper than my my specific band. I examined temperature gradient, different template concentration (template is vector...
11 December 2013 1,949 16 View
I work on eukaryotic gene, I want to clone and express it, I transform my recombinant plasmid to Ecoli(BL21), after induction, my protein does not express. I don't see my protein band in SDS...
11 December 2013 4,819 7 View
Approximate concentrations are require in compared with the WHO permissible limts
11 August 2024 2,723 1 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
Hey there, As a synthetic chemist delving into theoretical calculations for my imidazolium-based organic molecules, I would greatly appreciate any guidance on the appropriate input instructions...
09 August 2024 5,444 7 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View