I want to know how i can be certain about formation of inclusion body during protein expression?

I want to express a recombinant form of protein in bacteria and i'm not sure about this protein expression is soluble form. I want to know is there any way to be certain that it will be expressed...

08 September 2019 9,112 10 View

How can monitor how much studies are done on special matter?

I want to know when rate of application special kind of treatment started and how much studies exctlly are done on this matter . Actually is there any website to show rate of publication about...

08 September 2018 8,206 3 View

How can one reduce As+3 to As0 without interference of As+5?

01 February 2015 3,881 3 View

Can a circular dichroism monitor immobilization effect on protein structure?

31 December 2014 9,525 6 View

Can anyone help me with FTIR interpretation?

I immobilize enzyme on chitosan macrobead with Glutaraldehyde as crosslinker. I want to know how FTIR confirm immobilization? Which wavelength is related to chitosan with glutaraldeyde and which...

31 December 2014 2,603 11 View

Why doesn't my protein have desirable activity?

I work on cellulase enzyme and express it in E.coli(BL21). In SDS-PAGE I have good bands, but after purification with Ni-NTA column and dialysis, it's activity is very low (according to enzymatic...

05 June 2014 6,083 16 View

What is my problem in cloning?

I want to clone my gene to expreesion vector in Ecoli, after I clean my pcr product, I have a good band. In digestion step, after digesting the gene I don't get any band, but I perform a ligation...

01 February 2014 5,012 17 View

I do gel cracking for separate bands, unfortunately I only see a smear in each well. Why only the weak gene band is visible?

I reculture each colony in another plate after transformation and use them for cracking in the cracking buffer (SDS,NaOH,EDTA,D2O), I run them with 10 micro dye hardly but I don't see plasmid...

31 December 2013 8,203 1 View

Why is my PCR band is so weak?

I do PCR reaction for my gene (it's about1600bp). Nonspecific bands are sharper than my my specific band. I examined temperature gradient, different template concentration (template is vector...

11 December 2013 1,730 16 View

Can someone advise me why my protein fails to express?

I work on eukaryotic gene, I want to clone and express it, I transform my recombinant plasmid to Ecoli(BL21), after induction, my protein does not express. I don't see my protein band in SDS...

11 December 2013 4,682 7 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View