PR (Pathogen related) genes in N.tabacum?

Please help me finding sequence of the PR(Pathogen related) genes expressed during HR (hypersensitive response) in N.tabaccum, either acidic or basic. I need all. Thanks

07 August 2015 1,838 1 View

How can I locate the position of T-DNa insertion in a genomic structure?

I have SALK sequence no of a T-DNA mutant of a gene. now i want to make a genomic diagram with the position of insertion. How could I find the position of insertion. I check the SALK homepage but...

05 June 2015 8,322 5 View

What is COOR stands for in SALK T-DNA page?

05 June 2015 6,626 0 View

How can I isolate my plasmid from Agro GV3101?

I isolated my plasmid (includes my insert) from GV3101 by mini prep kit but the concentration is too low. is there any other method to isolate the plasmid from GV3101?

04 May 2015 2,613 9 View

Can someone help with protein extraction from N. benthamiana?

I extracted total protein from agro-infiltered N. benthamiana leaves by using following extraction buffer :- Protein Extraction Buffer- 50mM Tris/HCl pH 7.6, 100mM NaCl, 0.05% Tween20, 1mM EDTA,...

02 March 2015 5,690 6 View

How do I concentrate protein from lamelli buffer?

I already extracted my protein directly in SDS buffer containing Bromophenol blue and also B-mercaptoethanol. After running SDS page, I am having problem with protein concentration. Is there any...

31 December 2014 6,663 12 View

Does anyone know of a protocol that gives high yield of ER membrane and nuclei protein from Nicotiana benthamiana?

Please some one suggest me a good protocol, giving high yield of ER membrane and nuclear protein (if both protein extraction in same buffer, then better) from agroinfilterated Nicotiana...

31 December 2014 172 4 View

I am always getting a smear as a result of my SDS page. what may be possible reason to this?

SDS page troubleshooting.

10 November 2014 6,362 18 View

How can I carry out RACE for a known sequence?

06 July 2014 8,425 3 View

Can anyone help with fusion PCR?

I have two genes. I am isolating both of them (gel purification), then using each gene in one reaction of PCR with end primers for fusion of both genes, but each time I don't get any results for...

03 April 2014 2,585 26 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View