I did SDS-PAGE twice but my samples only ran half way through the gel including the stacking gel. But samples in another unit in the same tank ran properly.

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Monika Raina

Please be clear with with problem, not able to understand.

Is it that your gel has run half way only, if so pass voltage to allow it move to the other end.

Sri Harsha Meghadri

Sounds more like there is problem with casting of your gel...

Pratima Chawla

I ran two gels in one unit. One of them rum properly but other do not.. i used 100V voltage

Guruprasad Gopalakrishnan

I think there would be a problem in your casting or loading. Also check the wells for leakage.

Why dont you rerun the half run gel. I feel you have applied less voltage. We generally use 200V voltage for one small unit, and it takes it about 30min to run completely.

Thanks a lot to all. i will do it again. thanks.