Pratima Chawla

13 Questions 44 Answers 0 Followers

Questions related from Pratima Chawla

PR (Pathogen related) genes in N.tabacum?

Please help me finding sequence of the PR(Pathogen related) genes expressed during HR (hypersensitive response) in N.tabaccum, either acidic or basic. I need all. Thanks

08 August 2015 1,905 1 View

How can I locate the position of T-DNa insertion in a genomic structure?

I have SALK sequence no of a T-DNA mutant of a gene. now i want to make a genomic diagram with the position of insertion. How could I find the position of insertion. I check the SALK homepage but...

06 June 2015 8,412 5 View

What is COOR stands for in SALK T-DNA page?

when we go to SALK site, page opens like CLON SAIL.... TYPE SALK T-DNA CHRO chr3 EVAL 0.0 COOR C/16757979-16758431 what is COOR stands for. is this for the position of insertion of T-DNA

06 June 2015 6,713 0 View

How can I isolate my plasmid from Agro GV3101?

I isolated my plasmid (includes my insert) from GV3101 by mini prep kit but the concentration is too low. is there any other method to isolate the plasmid from GV3101?

05 May 2015 2,696 9 View

Can someone help with protein extraction from N. benthamiana?

I extracted total protein from agro-infiltered N. benthamiana leaves by using following extraction buffer :- Protein Extraction Buffer- 50mM Tris/HCl pH 7.6, 100mM NaCl, 0.05% Tween20, 1mM EDTA,...

03 March 2015 5,761 6 View

Does anyone know of a protocol that gives high yield of ER membrane and nuclei protein from Nicotiana benthamiana?

Please some one suggest me a good protocol, giving high yield of ER membrane and nuclear protein (if both protein extraction in same buffer, then better) from agroinfilterated Nicotiana...

01 January 2015 296 4 View

How do I concentrate protein from lamelli buffer?

I already extracted my protein directly in SDS buffer containing Bromophenol blue and also B-mercaptoethanol. After running SDS page, I am having problem with protein concentration. Is there any...

01 January 2015 6,726 12 View

I am always getting a smear as a result of my SDS page. what may be possible reason to this?

SDS page troubleshooting.

11 November 2014 6,471 18 View

How can I carry out RACE for a known sequence?

I know the sequence of my gene but want to do the RACE to confirm the position of 5 end prime and 3' end prime. Now, I am confused about how RACE works in this case and we can say by seeing at...

07 July 2014 8,503 3 View

Can anyone help with fusion PCR?

I have two genes. I am isolating both of them (gel purification), then using each gene in one reaction of PCR with end primers for fusion of both genes, but each time I don't get any results for...

04 April 2014 2,664 26 View

Does anyone have experience isolating bZIP28 of arabidopsis and its cloning?

I am willing to isolate bZIP28 transcription factor gene from arabidopsis, but having problem in extracting the gene.

04 April 2014 412 6 View

I want to check the T-DNA insertion mutant line bzip60, whether it is really a mutant or not?

How should I make primers to check that? I want to design primers to check the T-DNA insertion mutant line, want to learn from scratch.

10 October 2013 9,694 3 View

I did SDS-PAGE twice but my samples only ran half way through the gel including the stacking gel. But samples in another unit in the same tank ran properly.

Samples do not run

02 February 2013 3,777 6 View