PR (Pathogen related) genes in N.tabacum?

Please help me finding sequence of the PR(Pathogen related) genes expressed during HR (hypersensitive response) in N.tabaccum, either acidic or basic. I need all. Thanks

07 August 2015 1,838 1 View

How can I locate the position of T-DNa insertion in a genomic structure?

I have SALK sequence no of a T-DNA mutant of a gene. now i want to make a genomic diagram with the position of insertion. How could I find the position of insertion. I check the SALK homepage but...

05 June 2015 8,322 5 View

How can I isolate my plasmid from Agro GV3101?

I isolated my plasmid (includes my insert) from GV3101 by mini prep kit but the concentration is too low. is there any other method to isolate the plasmid from GV3101?

04 May 2015 2,613 9 View

Can someone help with protein extraction from N. benthamiana?

I extracted total protein from agro-infiltered N. benthamiana leaves by using following extraction buffer :- Protein Extraction Buffer- 50mM Tris/HCl pH 7.6, 100mM NaCl, 0.05% Tween20, 1mM EDTA,...

02 March 2015 5,690 6 View

How do I concentrate protein from lamelli buffer?

I already extracted my protein directly in SDS buffer containing Bromophenol blue and also B-mercaptoethanol. After running SDS page, I am having problem with protein concentration. Is there any...

31 December 2014 6,663 12 View

Does anyone know of a protocol that gives high yield of ER membrane and nuclei protein from Nicotiana benthamiana?

Please some one suggest me a good protocol, giving high yield of ER membrane and nuclear protein (if both protein extraction in same buffer, then better) from agroinfilterated Nicotiana...

31 December 2014 172 4 View

I am always getting a smear as a result of my SDS page. what may be possible reason to this?

SDS page troubleshooting.

10 November 2014 6,362 18 View

How can I carry out RACE for a known sequence?

06 July 2014 8,425 3 View

Can anyone help with fusion PCR?

I have two genes. I am isolating both of them (gel purification), then using each gene in one reaction of PCR with end primers for fusion of both genes, but each time I don't get any results for...

03 April 2014 2,585 26 View

Does anyone have experience isolating bZIP28 of arabidopsis and its cloning?

I am willing to isolate bZIP28 transcription factor gene from arabidopsis, but having problem in extracting the gene.

03 April 2014 345 6 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View