How can I locate the position of T-DNa insertion in a genomic structure?

I have SALK sequence no of a T-DNA mutant of a gene. now i want to make a genomic diagram with the position of insertion. How could I find the position of insertion. I check the SALK homepage but...

05 June 2015 8,322 5 View

What is COOR stands for in SALK T-DNA page?

05 June 2015 6,626 0 View

How can I isolate my plasmid from Agro GV3101?

I isolated my plasmid (includes my insert) from GV3101 by mini prep kit but the concentration is too low. is there any other method to isolate the plasmid from GV3101?

04 May 2015 2,613 9 View

Can someone help with protein extraction from N. benthamiana?

I extracted total protein from agro-infiltered N. benthamiana leaves by using following extraction buffer :- Protein Extraction Buffer- 50mM Tris/HCl pH 7.6, 100mM NaCl, 0.05% Tween20, 1mM EDTA,...

02 March 2015 5,690 6 View

How do I concentrate protein from lamelli buffer?

I already extracted my protein directly in SDS buffer containing Bromophenol blue and also B-mercaptoethanol. After running SDS page, I am having problem with protein concentration. Is there any...

31 December 2014 6,663 12 View

Does anyone know of a protocol that gives high yield of ER membrane and nuclei protein from Nicotiana benthamiana?

Please some one suggest me a good protocol, giving high yield of ER membrane and nuclear protein (if both protein extraction in same buffer, then better) from agroinfilterated Nicotiana...

31 December 2014 172 4 View

I am always getting a smear as a result of my SDS page. what may be possible reason to this?

SDS page troubleshooting.

10 November 2014 6,362 18 View

How can I carry out RACE for a known sequence?

06 July 2014 8,425 3 View

Can anyone help with fusion PCR?

I have two genes. I am isolating both of them (gel purification), then using each gene in one reaction of PCR with end primers for fusion of both genes, but each time I don't get any results for...

03 April 2014 2,585 26 View

Does anyone have experience isolating bZIP28 of arabidopsis and its cloning?

I am willing to isolate bZIP28 transcription factor gene from arabidopsis, but having problem in extracting the gene.

03 April 2014 345 6 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View