I am doing ELISA and it requires 4pl
Someone suggested me this but when I try to do -
fit
I read an interesting article from Christensen (2007): A Monte Carlo approach to unidimensionality testing in polytomous rasch models. But do you have some further thoughts or maybe other...
08 August 2024 4,245 0 View
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
05 August 2024 853 3 View
I conducted an adsorption experiment of arsenic on soil in the presence of different doses of silicon as competing ions to see the effect of silicon on arsenic adsorption and desorption. I took 5...
03 August 2024 6,498 3 View
The adsorbate is Arsenic and the adsorbent is a soil
18 July 2024 332 8 View
I am using a polygon mirror scanner that have 6 facets and the dc brushless motor speed was given 10,000 RPM. Now I want to know how to calculate the polygon speed if I change the power supply...
11 July 2024 5,941 1 View
I'm attempting to simulate a crossed dipole antenna. For excitation, I've placed a lumped port along each dipole. However, the resulting radiation pattern is similar to that of a single dipole....
08 July 2024 1,876 3 View
Does anyone know if there is a lab scale encapsulation machine available for purchase?
05 July 2024 3,887 1 View
I have used this BIorad Powerpac Basic for 4 years now, before which it was used for another 5-6 years. Generally we addressed the E1 error by compensating with fresh buffer and/or filling the...
01 July 2024 7,104 1 View
I am doing md simulation for protein aggregation. For doing cluster analysis I was trying with gmx clustsize. Now the plot that I am getting, can not find a proper explanation. With -hc flag the...
24 June 2024 5,245 0 View
if yes, is the publication free of charge
06 June 2024 4,270 1 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...
14 July 2024 6,724 0 View
Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?
23 June 2024 2,264 4 View
If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...
17 June 2024 6,704 1 View
I used two standards (one came with the ELISA kit and the other I made myself, by preparing a series of known concentrations of the target analyte) on the same plate in my ELISA run. Why are the...
15 June 2024 5,109 1 View
Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...
13 June 2024 9,472 0 View
We have to report the concentration in pg/ml, the ELISA kit provider did not reveal the specific activity of the enzyme. In that case how to convert the units?
05 June 2024 2,172 5 View
I have a question about AUC for calculating SARS-CoV-2 neutralizing antibody tests. AUC here is not the Area Under the ROC Curve. After six serial dilutions of human serum, an indirect ELISA was...
05 June 2024 8,191 0 View
I want to measure a protein in serum using an ELISA kit. This kit allows serum samples to be diluted 200 to 3200 times, but I want to use this kit to titrate higher concentrations of protein. Can...
03 June 2024 4,937 0 View
