I am looking for the protocol for DNA extraction from prostate biopsies?

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I cannot now see how to send a paper to the person who requested it. Please explain?

There sems to be a change in the program. It's not the same as before

22 January 2024 8,416 0 View

How would you qualitatively / quantitively analyse the amorphous phase(s) of glass beyond SEM-EDX analysis?

Can XRD-Rietveld analysis provide components / phases present in the amorphous phase the same way it does for crystalline material? Are there other methods available?

09 November 2023 7,044 1 View

Is there anyone I can contact, or any research papers on emergent behaviour in a simulated environment?

My friend is doing a folio for an assignment and needs information on this topic.

10 August 2023 7,675 6 View

Are there any experts I can contact that knows a lot about alcohol in the Middle East?

I am doing a folio on alcohol in the Middle East and I would like to interview a few people over email. Is there anyone you may know of that knows about alcohol in the Middle East? Things such as...

08 August 2023 3,158 2 View

How do I manipulate this phosphorus XANES data so that I can process it Athena?

I collected some data during my PhD for P L2,3 XANES, but the raw data was step like, meaning I could not import this quality of data into Athena to analyse. It has been a few years since I have...

02 August 2023 9,990 2 View

Why am I getting low Se82 recoveries on my ICPMS?

I run an Elan DRC II and a Nexion 1000 ICPMS. On both instruments, my calibration and all QC (including a second source standard) pass within 5% of 100 for Se82. However, when I run a sample...

16 April 2023 7,092 0 View

Does anyone have any recommendations on repeater pipettes?

I have used this repeater in the past and it's been my favorite: https://www.pipettes.com/brandtech-handystepr-705002-electronic-repeating-dispenser-110v-us-plug It's easy to use and has the...

05 February 2023 4,577 0 View

What centrifuge speed (in xg) will cause RBC to rupture?

We are doing a study where we have to collect blood and then spin down the sample twice, once at a low speed and once at a higher speed. We are trying to standardize centrifuge speeds between...

15 December 2022 8,661 0 View

Are there any free sample inventory programs for academic research?

Hello. I am a researcher at the University of Pittsburgh. In the past, we've primarily relied on physical binders to help us keep track of where our samples are inventoried. We have a huge...

01 November 2022 2,520 0 View

Anyone can suggest a research instrument to use in Job Performance descriptive research for Hospitality Industry?

I am working on my MBA -HROD paper, and I have chosen a Job Performance Topic in the hospitality industry. Your input is valid and appreciated. Sincerely, Katrina

21 April 2022 2,937 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View