How do you interpret and understand 11B 3Q MAS NMR graph?

I am a novice to NMR and need help in understanding what i am looking at in terms of 11B 3Q MAS NMR graphs. Any resources for beginners would be helpful in interpreting the data. Literature only...

20 January 2021 2,648 3 View

Is there a way to distinguish between overlapping energies in XAS graphs from the P L1-edge from the B K-edge?

Is there a post analytical methodology or experimental technique used to distinguish between overlapping energy edges in XAS?

09 September 2020 2,894 4 View

How do you apply chemometrics to Raman spectroscopy of glass samples with varying degrees of crystallinity?

What are the formula's and peak fitting types needed e.g. gaussian, lorenzian...

28 April 2020 2,301 3 View

Is there any other analysis methods one can do to analyse Raman spectroscopy data, other than stacking the plots and doing difference curves?

I am looking at glasses and the effect a certain component has on the peak positions and intensities. I do not have any complimentary techniques as of yet to help me analyse the data such as NMR.

27 April 2020 256 5 View

Which test to use in SPSS?

So i have a study with both males and females, they will both be shown a negative image and their brain reaction recorded and a positive image with their brain reaction recorded what analysis in...

30 March 2019 4,571 6 View

How to interpret and analyse data from accelerometer and gyroscope?

Hello, I am a research student looking into behaviour of horses. I am trying to use IMU devices to detect movement in horse movement (step frequency, lying down, etc). I understand how the sensor...

07 August 2018 5,646 3 View

What is the ideal procedure for testing anti-tumor with low cost price? What can you suggest procedure for extracting polysaccharides?

Hi! I am Katrina Co from the Philippines! I am just a High School student, I have only a limited samples for testing and a low budget (maybe around 6000 pesos or around $120). I only have limited...

29 May 2018 8,311 3 View

Any ideas for a protocol or extraction kit for extracting DNA from tomato fruit for the study of bacterial microbiota using NGS?

I'm trying to extract DNA from tomato fruit in order to study the bacterial microbiota found inside the fruit using NGS. I have tried using a CTAB protocol, but the precipitate is highly charged...

20 April 2018 423 3 View

Can anyone suggest a psychometrically valid, reliable, and quantitative measure of "Intentionality" attributions?

20 May 2015 5,982 2 View

How to check if the primer can attach to another organism from the same family?

I am working with two types of viruses from the same family. I was provided with specifically designed primers to test if either of the viruses are present in the material. However, I ran a PCR...

02 May 2014 4,866 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View