Dear Abid,

your question is too vague. Why??

you write :

I am doning a expriment for knowing about binding affinity with flouresence spectropholormeter

Binding affinity  of WHAT??

what a kind of  Fluo- spectrometer. What a kind of cuvettes and solvent.

Define "AFFINITY BINDING"  P.E.  LOW OR HIGH) AND  and  you must investigate the dissociation constants  in a certain range  a.s.o...

                                                    NOTE

that the answerers are not SLAVES  of questionners  for google searching and digging.!! Please don 't use acronym in a question  (NDM-1) or give the full name of NDM-1.

p.e.  

Novel fluorescent cephalosporins: Synthesis, antimicrobial activity and photodynamic inactivation of antibiotic resistant bacteria

JM Xiao, L Feng, LS Zhou, HZ Gao, YL Zhang… - European journal of …, 2013 - Elsevier

... of novel fluorescent cephalosporin TCA against K. pneumonia and fluorescence imaging of ...

Fluorescent cephalosporins; Antimicrobial activity; Photoinactivation; Substrate. ... β-lactamases

including recently reported New Delhi metallo-β-lactamase 1 (NDM-1) becoming resistant ...

Zitiert von: 7 Ähnliche Artikel Alle 4 Versionen Zitieren Speichern

Have you done a search with Google Scholar?

Insert   "Fluorecence life time value of NDM-1 protein"  in the mask of Google scholar.

You will obtain  about 500 hits.

PLEASE REFORMULATE YOUR QUESTION  and give infos about spectrometer and

substrate(s).

JRG