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Hi..... Please help me regarding my experiment. i am in very trouble period now to troubleshoot in my experiment but i am not got any result satisfactory . Actually i want to make S mutans cells...
15 September 2015 4,177 2 View
Why does my protein stay in the pellet of the lysate? I expressed a KPC-2 Protein in NP6 cells the vector is PqE-2. After lysis (8M urea in a tris buffer pH 8), the protein is in the pellet. I...
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