I am looking for a p53 pseudogene in mouse, but all I can find using blast is the real p53 gene. How can I blast against a specific chromosome? (#14)

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Abel Tan

u can open up the nucleotide entry for chromosome 14 assembly (NC_000080.6), then blast 2 seq on it with your p53.

Matej Lexa

For example, blastn takes these two options that can limit the search:

*** Restrict search or results

-gilist

Restrict search of database to list of GI's

* Incompatible with: negative_gilist, seqidlist, remote, subject,

subject_loc

-seqidlist

Restrict search of database to list of SeqId's

* Incompatible with: gilist, negative_gilist, remote, subject,

Nicole Wheatley

Matej, Abel,

Thank you, I'll try those out!