Abel Tan

3 Questions 40 Answers 0 Followers

Questions related from Abel Tan

How to sort a ER membrane protein to the plasma membrane?

Hi, I have this protein called G6PT (glucose 6 phosphate translocase). Its a transmembrane channel.Natively it sorts to the Endoplasmic Reticulum membrane. I am doing some synthetic biology and...

24 July 2018 1,586 4 View

What are typical secreted protein Yields in Mammalian Cells?

Hi, Say I have some (untested)cancer cell line isolated from a patient. I clone in a arbitrary secretable gene into the strongest promoter and use the strongest RBS. I use a high copy number...

12 June 2017 1,398 2 View

How do I titer integration defective lentivirus using puromycin?

Hi, I have a puromycin casette transfected into an integration defective lentivirus vector. Normal (integration competent) lentivirus titer assays expects the user to count surviving cells after 3...

06 June 2017 6,484 3 View