I received a construct to be used with the Flp-In T-Rex system but the host cell line I need for my experiments (MDCK or any cyst-competent cell lines) is not commercially available.
This may be outdated at this point, but if you're still looking it may be worth checking here (doi:10.1152/ajprenal.90406.2008).
So I am organizing an AI seminar. What are possible AI projects in the AI for good spirit? something the students can do and have an impact?
08 August 2024 9,437 4 View
Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?
23 June 2024 2,264 4 View
Need help. concerning the electroanalysis of tramadol, by varying the pH, I obtained according to the equation on the right a slope of -0.00121 mv. Is it possible? It is true that at different pHs...
04 May 2024 267 0 View
Hi everyone, I want to study the translation efficiency of multiple version of the same protein coded by different genotypes. To do so I need to conserve the translation initiation context ->...
29 April 2024 6,779 1 View
When I click on the blue tab "Add text publicly", nothing happens. So I try the upper right tab "Add new"->published research->article, then click "Select and upload file". Nothing happens,...
28 April 2024 9,341 8 View
I am interested on the application of Christian esotericism and Hermeticism to the development Of Orthodox Theology and spirituality (Sophiology, Theosis, theological anthropology). I am also...
25 March 2024 2,449 6 View
Often I need to transform several columns of data into rows of aligned values in a matrix, for multivariate analysis. So randomly ordered values in the same sample are arranged/aligned into the...
13 March 2024 6,026 1 View
Hi everyone, everything is in the title question ! To the best of your knowledge, what is the shortest sequence you know that translate into a fluorescent protein that could be used for flow...
07 March 2024 1,762 1 View
Hi everyone, I'm currently working on the HaCaT keratinocyte cell line. I have them in culture under low and high calcium concentration to keep them undifferenciated or differenciated,...
04 March 2024 2,153 0 View
Hello everyone, I use several pcDNA3.1 expression vectors to transfect cells. The vectors were prepared by midi-prep a year ago and diluted in TE buffer. Now that I run new experiments, I...
04 March 2024 1,867 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View