Why my deltaATG mRNA is in polysome fractions ?

Hello everyone, I'm doing polysome profiling to answer a specific question. I study a gene which produce an alternative transcript and it's translation is controversial (extremely lucky if you...

16 December 2020 7,622 2 View

Interesting BuLi reactions?

Dear colleagues! At the moment we are looking for "nice" reactions employing BuLi solutions for our practical training at the university. We aim to expand our five standard BuLi reactions to a...

08 December 2020 6,481 10 View

Are the particles well dispersed based on my results below?

What I want to do: I have a colloidal dispersion which comprises silicon dioxide (SiO2) particles (diameter=450nm) and poly(ethylene glycol) and want to confirm that the SiO2 particles are well...

16 November 2020 817 16 View

Methodology Design Feasibility / Ideas for Methodology?

Hi, I am trying to come up with a methodology to better understand how effective are SETs at evaluating faculty performance in the classroom? I was thinking about a design in which a faculty...

09 November 2020 156 3 View

The response rate of the highly cited journals to the topic COVID-19?

Dear member, Does anybody knows that Highly cited Journals is faster responding to submission with COVID-19 topic (Especially Economics Journals), and what is the average time for response? I...

26 October 2020 7,888 8 View

Do you know of a researchable topic within course evaluations?

Hi, I am writing my dissertation and I am doing it on course evaluations. I have always wondered what happens to them after students click "submit". Do they inform practice? I believe that if...

11 October 2020 2,634 4 View

Any advice on running quantum espresso on a single Imac??

I have been using VASP for years now and wanted to try out QE on my home IMac since it's open source. Does anyone have any advice?? I recently wiped and reinstalled the OS on the mac so it is...

29 September 2020 5,195 2 View

Does polysome profiling in 4mL tube instead of 12 mL needs a scale down in ultracentrifugation time ?

Hello everyone, I recently started several polysome profiling experiment and something bugs me out. Every protocols I found use 12mL tube. They load their lysate containing ribosomes attached to...

19 September 2020 8,717 10 View

Do you know of publications reporting fisheries post-release survival rates of marine turtles?

We are compiling publications that estimate marine turtle survival following release from fishing gear. We've identified a handful of relevant pubs and hope to identify a few more.

17 September 2020 3,194 6 View

How can I save one image from an image stack?

Hi, I've received a TIF file comprising 20 images (yes, 20 images compiled in one TIF file).. I can view the 20 images with windows photo viewer but I can't save the image I want.. Please help me....

06 August 2020 5,043 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View