Why my deltaATG mRNA is in polysome fractions ?

Hello everyone, I'm doing polysome profiling to answer a specific question. I study a gene which produce an alternative transcript and it's translation is controversial (extremely lucky if you...

16 December 2020 7,622 2 View

HowScholarships, Fellowships and grants in Materials engineering for international students to study abroad?

On Line student doctorant looking for first Master PhD student doctorant as UNICAF now in Materials engineering for international students to study abroad on Line and To Bricks LTGS and To have...

10 December 2020 3,686 3 View

Merging two same papers in a one paper?

Hello, I have three same papers which have different numbers of citations and different number of readings and I would like to merge these three papers in one paper with taking account of a new...

20 September 2020 5,275 6 View

Does polysome profiling in 4mL tube instead of 12 mL needs a scale down in ultracentrifugation time ?

Hello everyone, I recently started several polysome profiling experiment and something bugs me out. Every protocols I found use 12mL tube. They load their lysate containing ribosomes attached to...

19 September 2020 8,717 10 View

What is the sensitivity of SHM technique based on electromechanical impedance method to identify a crack in a concrete beam?

The sensitivity of the technique is important to know how to identify the appearance of cracks

17 August 2020 4,156 9 View

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31 May 2020 6,077 6 View

How can you efficiently collect a ground-nesting bee when it is inside its nest?

I am looking for an efficient way to collect a ground-nesting bee at its nest, so that when encountering a ground-nesting bee nest, I could determine what species created that nest. Ideally, I was...

21 February 2020 5,269 3 View

What control do you need for polysome profiling experiment ?

Hi everyone, I was wondering what antibodies or pcr primers I would have to use to control my fractions in a polysome profiling experiment ? A quick search highligthed antibodies against small...

09 October 2019 4,688 1 View

Does someone has an interest for XIXth century herbaria prepared in Brazil?

While investigating about French women botanists in the XIXth Century I chanced upon two herbarias, made from plants collected in Brazil. I am looking for someone who could help to evaluate the...

07 October 2019 2,560 6 View

Journals to submit a method to assess Vulnerability of Hotels to Earthquakes ?

Dear all, What are the most appropriate journals to submit a method we developed to estimate the seismic vulnerability index for touristic accommodations ? and its application to a pilot zone of...

08 September 2019 8,878 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

16 February 2021 9,050 5 View

Gromacs force field and water model recommended for protein hydrophobic study?

Dear All, I received a comment from reviewer about asking me selection of force field and water model. I choose OPLS-AAL and 3-point water model. They mentioned the combination of OPLS-AAL and SPC...

13 February 2021 1,364 3 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View