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I received a construct to be used with the Flp-In T-Rex system but the host cell line I need for my experiments (MDCK or any cyst-competent cell lines) is not commercially available.
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I'm currently growing Caco-2 cells in T-Flask before transferring them to a Matrigel embedded system. The problem is that obviously these cells don't like the plastic and grow in big groups of...
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