I am using Ovcar8 and A1847 as my methylated cell lines and now I need to add one more cell line in-vitro for the further clarification of my results .
Hi Tahira, breast cancer MDA-MB-231 and MCF-7 cell lines show BRCA1 gene methylated on different sites. Easy to get as well as to culture and eventually use in vivo experiments. The first one is a triple negative, highly metastatic, while the second one is ER+ and PR+, and no metastatic. Thus you may end up having nice results from quite opposite sides of breast cancer. Have a look at the paper attached for further info. Good luck!
hi Tahira, you can get from University Of Santa Thomas, Manila in Philippines, that is where i got cell lines i am using and they have different cell lines BRCA 1 AND 2. hope i was able to help. goodluck