I am looking for a buffer system maintaining a pH range of 6.1 – 6.7 which does not interact with the metabolism of yeast cells included in the reaction solution (positive and negative interaction).
Succinate buffer might do the trick. Check the link below:
Article Trait Variation in Yeast Is Defined by Population History
I think BIS-Tris buffer can be useful here. It is often used in buffered culture media for bacteria, yeast and mammalian cells.
I have used maleic acid and Tris in order to get a Tris/maleate solution to buffer effectively a pH range between 6.2 - 6.8 and tested in respiration assays for yeast isolated mitochondria. To my knowledge, maleate is not metabolized in yeast and it cannot interconvert to other metabolites; such as malate, fumarate or succinate spontaneously. Therefore, I'd suggest to give it a try. It is also not expensive.
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