6 Questions 18 Answers 0 Followers
Questions related from Shoyab Ansari
Hi Is there any way to remove/separate all the membrane lipid components from the HEK cell lysate?
25 June 2023 3,273 1 View
Hi All, I am using cell mask orange dye to label HEK 293 T cells plasma membrane. I am using 5 ug of dye as final concentration and incubating cells for 10 min at 37•C. I am facing issue with dye...
31 January 2023 7,521 7 View
I did SDM.After that i run pcr product on gel and i did get amplification.(Attached pic).Then i did Dpn-1 digestion followed by the gel run again.now this time i did nt get any band and Gel was...
30 March 2019 9,788 4 View
I am doing SDM by stratagene kit.After PCR amplify product was observed on agarose gel.Beside that i run my original plasmid on gel too.Unfortunately after PCR i was not getting correct amplify...
08 March 2019 6,324 7 View
how much concentration of tcep require to reduce the protein without interfering the maleimide cysteine conjugation reaction?
05 May 2018 5,906 7 View
texas red maleimide (5mg)-i am using 10mM stock solution.For how many day i can use this dye stock solution??please suggest me?
19 April 2018 9,204 3 View
