I am having problems in isolating good quality RNA from plant tissue? I am attaching nano drop reading for your reference?

02 December 2024 0 5K Report

Can anyone suggest the reason for lower values of 260/230 in nano drop reading for RNA from plant samples.

More Ritika Vishnoi's questions See All

Content Analysis tool for risk disclosure in annual reports?

hi, can someone help with possible ways of doing content analysis from annual reports of Indian companies. I am looking at risk disclosure practices of companies and how can i create a risk...

13 July 2023 1,075 3 View

CRISPR Cas9 based single nucleotide change in rice?

Can anyone tell me is there any way by which a single base can be changed in rice by using CRISPR Cas9

01 April 2023 5,222 0 View

During conventional stickey end cloning, dies the ratio of vector to insert depends on the size of the vector and insert?

I am trying to clone h 533 bp fragment in a 21000bp vector. What should be appropriate ratio of vector to insert to use for successful cloning.

31 December 2022 6,736 3 View

Which polymerase is good for amplifying 4.5kb fragment from a plasmid?

28 December 2022 9,065 5 View

What is the maximum size of amplicon amplified by pfu polymerase?

I want to amplify a 4.5kb fragment from a plasmid. Can pfu polymerase from genebio is suitable for the job?

28 December 2022 1,412 3 View

How to lyophilize isolated DNA sample in a simple way?

I have DNA isolated from Leishmania parasites using a Qiagen kit. Please guide me how to lyophilize this DNA sample.

08 November 2022 292 2 View

Is it necessary to use T4 polynucleotide kinase during annealing oligos for guide RNA cloning?

I am having difficulties in ligating guide RNA in a vector, digestion happens but ligation doesn't. I am not using T4 polynucleotide kinase during oligo annealing ( Zhang's lab protocol). So, I...

24 September 2022 7,248 0 View

Does anyone know GFP fusion with gene of interest vector for wheat?

I am looking for a vector which allows translational fusion of gene of interest with GFP for wheat. Does anyone know about any such vector.

13 September 2022 6,204 0 View

Golden Gate cloning using Bbs1 not working?

I am trying to assemble 5 different construct in a single destination vector but my digestion ligation reaction is not working. I am getting all blue colonies except one or two white colonies. By...

10 July 2022 359 0 View

What is the formula to calculate Canopy cover % ? We have diameter at breast height, crown area of individual trees, total number of trees, and area ?

I am looking for a good formula to compute % canopy cover for the trees of a plot. I have the dbh for all the trees, calcualted crown projection area (using the crown radius of the species),...

10 May 2022 1,939 0 View

Nano Orange protein quant on the Quantus fluorometer?

Is anyone here using the Thermo Fisher Nano Orange kit for protein quantification, and detecting the fluorescent signal on the Promega Quantus? We are struggling to make sense of the standard...

05 August 2024 8,946 3 View

Which is true nano nitrogen or nano urea?

Some scientists use nano nitrogen and some use nano urea, even though the process used the same for preparation. So, which is true nano nitrogen or nano urea?

28 June 2024 2,885 6 View

Any specific peptide based ligand for mitochondrial targeted delivery of nano-drug delivery systems?

Suppose I need to target mitochondria to address malignancy-related problems. Are there peptide-based ligands similar to Nuclear Localization Signals (NLS) for nuclear targeting that can be used...

20 June 2024 9,587 1 View

How can I select the narrow band filter in Zetasizer nano ZS?

I am working with chitosan/dextran sulphate nanoparticles loaded with rifampicin, which is fluorescent. Dsl analysis are bad and I saw that is necessary use a narrow band filter. How can I select...

05 June 2024 1,324 3 View

Novel and future lubricants and additives for hybrid electric vehicles?

nano additives and lubricants for hybrid electric vehicles

24 May 2024 8,363 3 View

Can we make aqueous or ethanol extraction or by other solvent extraction in Nano size? which method that used for it if it possible please?

can we make aqueous or ethanol extraction or by other solvent extraction in Nano size? which method that used for it if possible, please?

19 May 2024 9,308 1 View

Using a Jetson Nano with Waveshare UPS Module B in a car needing 8.4V/2A. How can I charge it using the car's system?

Hi everyone, I'm currently working on a project where we are using a Jetson Nano in a car, supported by a Waveshare UPS Module B for battery backup. To charge the UPS Module, we need a supply of...

19 May 2024 205 3 View

Are you intersted in nanoplastic detection?

to be involved in our research please massage me here

15 May 2024 8,998 2 View

X-ray diffraction peak at 80 degrees?

The above is the X-ray diffraction diagram from the iron nano sample that I synthesized from green tea extract. Could you please explain the characteristic peak at the 80-degree position?

03 May 2024 4,172 6 View

Is this possible to make Nano Crystalline Cellulose (NCC) from Micro Crystalline Cellulose (MCC) of Jute Fiber?

I want to study the properties of Jute NCC. What are the changes, if I make this nano to micro?

01 May 2024 7,385 4 View