Transformers

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Are my yeast competent cells bad (K. lactis Protein Expression Kit (NEB))? | ResearchGate

I am having difficulty in obtaining transformants, the positive control plasmid pKLAC2(malE) was prepared for transformation and only small colonies were observed. These did not secrete malE...

01 January 1970 5,359 0 View

Is charring of a compound while measuring its melting point, can be considered the melting point? | ResearchGate

I was measuring the melting point of a compound and I didn't see it changing to liquid instead it charred, I regarded the time when it charred as the melting point, is that correct? And why did it...

01 January 1970 3,622 0 View

What should be the incubation time for ligation reaction while using Thermo Scientific™ Rapid DNA Ligation Kit Catalog number: K1422? | ResearchGate

Hello everyone, I want to know what should be the optimum incubation time for ligation? In our lab, we are using Thermo Scientific™ Rapid DNA Ligation Kit Catalog number: K1422 for ligation, the...

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After transforming E.coil with the plasmids ,how can we identify the cells that contain a chimeric plasmid? | ResearchGate

After transforming E.coil with the plasmids ,how can we identify the cells that contain a chimeric plasmid?

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How to improve restriction-ligation cloning streategy ? | ResearchGate

Hello researchers, I am currently trying to generate a bank of mutants of a yeast gene I am working on. After performing a error-prone PCR based on dNTPs imbalance, I clone the mutated fragments...

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How to compare the strength of two Spearman's rho? | ResearchGate

I have 215 samples, and there are 9 independent variables. I want to know which independent variables could better predict the DV. However, the 9 IVs are highly correlated with each other, so I...

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How to transform output variables in DEA when outputs should be reduced instead of increased? | ResearchGate

I want to apply DEA to measure the efficiency of different DMUs regarding an undesirable output that should be reduced instead of increased. Meaning DMUs with minimal output should be classified...

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Will engineered bacteria have the same survivability as the unengineered ones in the same environment? | ResearchGate

If I will be integrating or transforming a soil bacteria strain, will the bacterial survivability remain the same as its unmodified version?

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