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I am having difficulty in obtaining transformants, the positive control plasmid pKLAC2(malE) was prepared for transformation and only small colonies were observed. These did not secrete malE...
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I was measuring the melting point of a compound and I didn't see it changing to liquid instead it charred, I regarded the time when it charred as the melting point, is that correct? And why did it...
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Hello everyone, I want to know what should be the optimum incubation time for ligation? In our lab, we are using Thermo Scientific™ Rapid DNA Ligation Kit Catalog number: K1422 for ligation, the...
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After transforming E.coil with the plasmids ,how can we identify the cells that contain a chimeric plasmid?
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Hello researchers, I am currently trying to generate a bank of mutants of a yeast gene I am working on. After performing a error-prone PCR based on dNTPs imbalance, I clone the mutated fragments...
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I have 215 samples, and there are 9 independent variables. I want to know which independent variables could better predict the DV. However, the 9 IVs are highly correlated with each other, so I...
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I want to apply DEA to measure the efficiency of different DMUs regarding an undesirable output that should be reduced instead of increased. Meaning DMUs with minimal output should be classified...
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If I will be integrating or transforming a soil bacteria strain, will the bacterial survivability remain the same as its unmodified version?
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