I am going to isolate leukocytes from milk of dairy cattle using either Histopaque or Ficoll. Does any of you have experiences with this isolation?

More Priscila Assis Ferraz's questions See All

About SUMO-TraCi interface: how to generate an increasing number of vehicles to simulate a congestion? How to access the simulation results in python?

I would like to simulate a congestion scenario using SUMO-TraCi interface. I was able to create the network layout and a few cars. How to generate an increasing number of vehicles moving at...

20 November 2023 1,907 1 View

How to prevent the loss of monocytes derived from PBMCs after PBS wash?

I seeded PBMCs in a 10 cm petri dish (1x10^8) and treated them with hM-CSF and incubated them at 37C with 5% CO2 for 3 days. After 3 days, I carefully added PBS to wash and lost most of my cells....

12 November 2023 2,523 5 View

Hi: SCP whenever I try to define an ROI in the training input I get the error: "Error [65]". Any idea what is causing this?

Error [65]: Error, extent of vector too large or attribute table error." I uninstalled and reinstalled QGIS 3.26.2 but I still get the error. Any idea what is causing this?

07 August 2023 4,797 1 View

I would like access to this book "Encyclopedia of Animal Cognition and Behavior" Does anyone have access to this download?

Encyclopedia of Animal Cognition and Behavior Editors: Jennifer Vonk, Todd Shackelford

06 June 2023 4,242 0 View

How to decrease the amount of white blood cells obtained from a murine hepatocyte extraction?

As a final project for a college course, we need to do a primary culture of non parenquimatic liver cells from mice. In our first try we obtained a great amount of white blood cells instead of...

14 May 2023 9,576 2 View

There are specific culture cell medium for in vitro culture of different primary tumor cells?

I want to grow (in vitro) primary cells from different types of pediatric tumors. It is known that fetal bovine serum induces cell differentiation in embryonic tumors, mainly. What is the most...

21 September 2022 1,040 0 View

Extration of urease from Cryptococcus neoformans, how keep the enzymatic activity ?

We are used the protocol for urease extration adaptated (to C. neoformans) from Amin et al.,2013: Briefly, the broth cultures were subjected to centrifugation (5,000 × g, 4 °C) and the...

25 November 2021 4,404 2 View

I produced a chimeric antibody in HEK293T cells. Why the SDS-PAGE of purified chimeric antibody resulted in two bands for the heavy chain?

In order to produce a chimeric antibody, I transformed HEK293T cells with two plasmids: one for heavy chain and the other for light chain IgG. The supernatant was purified in a Protein G-Sepharose...

28 March 2021 4,711 8 View

PDX leukemia in immunodeficient mice show regression after a time. Anyone knows what is going on?

I am performing PDX ALL in NSGS mice, but something curious happens: some leukemias develop very well , but after a time the hCD45+ cells show a huge regression while the mCD45+ cells turn back to...

16 December 2020 7,505 2 View

About the mouse humanization process, why should I wait between four to 24 hours after irradiation to transplant the stem cells into pups?

The protocol for the humanization of the NSG mice with human CD34+ cells demands that mice should be irradiated before the inoculation of the human cells. However, after this procedure, it is...

01 September 2020 8,630 4 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I improve the quality of lamb milk replacer for machine feeding?

Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...

06 August 2024 6,227 2 View

I found the zeta potential of exosomes in DLS and it was -8mv. So is it correct or not ?

What should be the zeta potential of bovine milk exosomes

25 July 2024 7,376 5 View

Why not cow dung manure used in lie for artificial ?

Cow dung manure is more powerful than artificial fertilizer.

19 July 2024 7,392 7 View

Is cow dung can be in used for repeated use ?

Is this organic cow dung manure now important than artificial fertilizer.if good then why ?

19 July 2024 9,052 2 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

How to make chocolate milk ?

we want to produce chocolate milk industrally .Dose anyone have a specific stabilizer formula for this pourpose?

10 July 2024 3,791 1 View

Many scientists have proven that the use of antibiotics against pathogens in dairy cows has a low success rate. What is the solution ?

I think that in such situations, hygiene measures are very important and the right milking machine is required. Which is the right one to obtain clean and safe milk with a high quality standard?

10 July 2024 4,362 0 View

How do I make plant based milk from cassava?

How do I make plant-based milk with cassava, corn, and potato starch? There is potential for starch.

02 July 2024 7,017 1 View

How can I analyze thermal camera images to measure heat stress in dairy cows?

Hi, I am working on heat stress in dairy cows. In a recent experiment, we used a FLIR-T62101 thermal camera to take photos of the cows. One photo is attached. How can I analyze these images to...

30 June 2024 2,190 5 View