I am getting non-specifc products in pcr by decreasing the mgcl2 conc.

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Why Lactobacillus acidophillus is sensitive to Vancomycin but L. rhamnosus and casei are intrinsically resistant?

Does it have, anything to do with their fermentation status? I know that in several species of LAB, the terminal d-alanine residue is replaced by d-lactate or d-serine in the muramylpentapeptide,...

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Modal Analysis Results - Ansys Workbench?

Hello, i had modeled a steel beam of dimensions as Length = 4000 mm, Cross section = 250 x 150 mm, E=2E+11 N/m^2, Density = 7850 kg/m^3. Boundary conditions: Left support is applied using Remote...

11 January 2020 9,945 4 View

How to perform k-fold cross validation for Neural Network in IBM SPSS Statistics?

I am Using IBM SPSS Statistics for Neural Networks but I am facing difficulty in cross validation of Model. Kindly suggest how to perform K-fold validation in SPSS Statistics. Thanks

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If we gene clean a pcr product having three different sequences in the middle of fragment but rest of sequence is same and they have same size

can any one tell that is it possible to differentiate between the three sequences (having same size) and obtain their individual sequence in the same sequencing reaction and if yes then what is...

06 June 2017 9,826 5 View

I have to perform a PCR by a reverse primer which have a mismatch, five nucleotide before the 3' end. How could it affect my PCR?

will it effect the efficiency of the primers or its specificty???

17 April 2017 9,315 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

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fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

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Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

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