I am getting non-specifc products in pcr by decreasing the mgcl2 conc.

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Nallapalle Sateesh Reddy

Non specific bands may appear in fallowing conditions

1.High concentration of MgCl2,

2.High concentration of primer

3. Low Annealing temperature

4.Less Annealing time

5. Long extension

Some other useful links to PCR Trouble shoot are

1. NEB: www.neb.com/tools-and-resources/troubleshooting-guides/pcr-troubleshooting-guide

2.Biorad: www.bio-rad.com/en-in/applications-technologies/pcr-troubleshooting