Mufarrah Mehboob

3 Questions 5 Answers 0 Followers

Questions related from Mufarrah Mehboob

If we gene clean a pcr product having three different sequences in the middle of fragment but rest of sequence is same and they have same size

can any one tell that is it possible to differentiate between the three sequences (having same size) and obtain their individual sequence in the same sequencing reaction and if yes then what is...

07 June 2017 9,904 5 View

I have to perform a PCR by a reverse primer which have a mismatch, five nucleotide before the 3' end. How could it affect my PCR?

will it effect the efficiency of the primers or its specificty???

18 April 2017 9,374 3 View

I am getting non-specifc products in pcr by decreasing the mgcl2 conc.

though we expect this to occur by increasing the mgcl2 conc...is it possible?? and if it is then how??

01 April 2017 7,168 3 View