Which are the latest technologies a Clinical Microbiology laboratory should have for rapid diagnosis of infectious diseases in a hospital?

Need to upgrade our laboratory. To provide a timely diagnosis of various bacterial, viral, fungal and mycobacterial infections and do epidemiological typing of various nosocomial pathogens.

02 March 2018 137 4 View

How do we remove amplicons from micropipettes?

My negative control is showing bands as in positive control. I repeated the experiment with new reagents and new primers. However the pipette used for adding template was used for gel loading also...

01 February 2016 5,052 4 View

Can anybody provide me a reference for speciation of filamentous fungi?

I want a reference on speciation of fungi especially zygomycetes.

11 December 2014 642 7 View

Why am I not getting bands in 1.8kb PCR for atypical mycobacteria?

I am getting a 1.8kb sized band in 1.8kb PCR encoding 16S-23S rRNA spacer region and their flanking parts with a reference H37 Rv strain but with none of the rapidly growing mycobacteria. What...

10 November 2014 9,295 5 View

Why do the bands towards the electrodes move slowly in my electrophoresis unit?

09 October 2014 5,444 11 View

Which is the shortest and most reliable DNA extraction protocol for MTB from clinical samples?

Most of the protocols and even the commercial ones are time consuming.

07 August 2014 4,292 3 View

How good is the boiling method / sonication for DNA extraction from Enterococci?

I have tried both methods without any success.

06 July 2014 4,852 4 View

Is anybody growing H.pylori on culture and doing its antibiotic susceptibility. Can you share your experience?

I am in the process of collecting biopsies for the same.

03 April 2014 9,761 31 View

Which is the current best and easy PCR based method to speciate yeasts?

I am doing PCR-RFLP to identify yeasts. I want to do away with the steps of RFLP. I know of a multiplex PCR using 3 primers for identification.

03 April 2014 2,737 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View