Gulnaz Bashir

10 Questions 32 Answers 0 Followers

Questions related from Gulnaz Bashir

Which are the latest technologies a Clinical Microbiology laboratory should have for rapid diagnosis of infectious diseases in a hospital?

Need to upgrade our laboratory. To provide a timely diagnosis of various bacterial, viral, fungal and mycobacterial infections and do epidemiological typing of various nosocomial pathogens.

03 March 2018 206 4 View

I am getting a non specific band in PCR product using ITS3 and ITS4 at annealing temperatures of 56,57,58 and 59oC. Can anybody help?

09 September 2016 4,979 3 View

How do we remove amplicons from micropipettes?

My negative control is showing bands as in positive control. I repeated the experiment with new reagents and new primers. However the pipette used for adding template was used for gel loading also...

02 February 2016 5,104 4 View

Can anybody provide me a reference for speciation of filamentous fungi?

I want a reference on speciation of fungi especially zygomycetes.

12 December 2014 716 7 View

Why am I not getting bands in 1.8kb PCR for atypical mycobacteria?

I am getting a 1.8kb sized band in 1.8kb PCR encoding 16S-23S rRNA spacer region and their flanking parts with a reference H37 Rv strain but with none of the rapidly growing mycobacteria. What...

11 November 2014 9,348 5 View

Why do the bands towards the electrodes move slowly in my electrophoresis unit?

I have recently purchased a new electrophoresis unit. On electrophoresis in 1 and 2% agarose, gel bands of same size on the electrode side move slowly giving the whole thing a slanting look. The...

10 October 2014 5,544 11 View

Which is the shortest and most reliable DNA extraction protocol for MTB from clinical samples?

Most of the protocols and even the commercial ones are time consuming.

08 August 2014 4,346 3 View

How good is the boiling method / sonication for DNA extraction from Enterococci?

I have tried both methods without any success.

07 July 2014 4,902 4 View

Which is the current best and easy PCR based method to speciate yeasts?

I am doing PCR-RFLP to identify yeasts. I want to do away with the steps of RFLP. I know of a multiplex PCR using 3 primers for identification.

04 April 2014 2,787 2 View

Is anybody growing H.pylori on culture and doing its antibiotic susceptibility. Can you share your experience?

I am in the process of collecting biopsies for the same.

04 April 2014 9,829 31 View