I am getting a non specific band in PCR product using ITS3 and ITS4 at annealing temperatures of 56,57,58 and 59oC. Can anybody help?

I am getting a non specific band in PCR product using ITS3 and ITS4 at annealing temperatures of 56,57,58 and 59oC. Can anybody help?

08 September 2016 4,922 3 View

How do we remove amplicons from micropipettes?

My negative control is showing bands as in positive control. I repeated the experiment with new reagents and new primers. However the pipette used for adding template was used for gel loading also...

01 February 2016 5,052 4 View

Can anybody provide me a reference for speciation of filamentous fungi?

I want a reference on speciation of fungi especially zygomycetes.

11 December 2014 642 7 View

Why am I not getting bands in 1.8kb PCR for atypical mycobacteria?

I am getting a 1.8kb sized band in 1.8kb PCR encoding 16S-23S rRNA spacer region and their flanking parts with a reference H37 Rv strain but with none of the rapidly growing mycobacteria. What...

10 November 2014 9,295 5 View

Why do the bands towards the electrodes move slowly in my electrophoresis unit?

09 October 2014 5,444 11 View

Which is the shortest and most reliable DNA extraction protocol for MTB from clinical samples?

Most of the protocols and even the commercial ones are time consuming.

07 August 2014 4,292 3 View

How good is the boiling method / sonication for DNA extraction from Enterococci?

I have tried both methods without any success.

06 July 2014 4,852 4 View

Is anybody growing H.pylori on culture and doing its antibiotic susceptibility. Can you share your experience?

I am in the process of collecting biopsies for the same.

03 April 2014 9,761 31 View

Which is the current best and easy PCR based method to speciate yeasts?

I am doing PCR-RFLP to identify yeasts. I want to do away with the steps of RFLP. I know of a multiplex PCR using 3 primers for identification.

03 April 2014 2,737 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View