Which are the latest technologies a Clinical Microbiology laboratory should have for rapid diagnosis of infectious diseases in a hospital?

Need to upgrade our laboratory. To provide a timely diagnosis of various bacterial, viral, fungal and mycobacterial infections and do epidemiological typing of various nosocomial pathogens.

02 March 2018 137 4 View

I am getting a non specific band in PCR product using ITS3 and ITS4 at annealing temperatures of 56,57,58 and 59oC. Can anybody help?

I am getting a non specific band in PCR product using ITS3 and ITS4 at annealing temperatures of 56,57,58 and 59oC. Can anybody help?

08 September 2016 4,922 3 View

How do we remove amplicons from micropipettes?

My negative control is showing bands as in positive control. I repeated the experiment with new reagents and new primers. However the pipette used for adding template was used for gel loading also...

01 February 2016 5,052 4 View

Why am I not getting bands in 1.8kb PCR for atypical mycobacteria?

I am getting a 1.8kb sized band in 1.8kb PCR encoding 16S-23S rRNA spacer region and their flanking parts with a reference H37 Rv strain but with none of the rapidly growing mycobacteria. What...

10 November 2014 9,295 5 View

Why do the bands towards the electrodes move slowly in my electrophoresis unit?

09 October 2014 5,444 11 View

Which is the shortest and most reliable DNA extraction protocol for MTB from clinical samples?

Most of the protocols and even the commercial ones are time consuming.

07 August 2014 4,292 3 View

How good is the boiling method / sonication for DNA extraction from Enterococci?

I have tried both methods without any success.

06 July 2014 4,852 4 View

Is anybody growing H.pylori on culture and doing its antibiotic susceptibility. Can you share your experience?

I am in the process of collecting biopsies for the same.

03 April 2014 9,761 31 View

Which is the current best and easy PCR based method to speciate yeasts?

I am doing PCR-RFLP to identify yeasts. I want to do away with the steps of RFLP. I know of a multiplex PCR using 3 primers for identification.

03 April 2014 2,737 2 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Any specific reason, how sclerotium rolfsii can grow out of Petridish?

Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?

09 February 2021 4,357 4 View

Where can I find Plasmopara viticola?

Hello everyone, For my research I want to infect Vitis Vinifera leaves with downy mildew-causing fungus Plasmopara viticola. Does anyone know where I could find or purchase this fungi? I am...

04 February 2021 5,720 3 View