How good is the boiling method / sonication for DNA extraction from Enterococci?

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Dr. Godfred A. Menezes

Boiling method (Colony PCR) is recommended usually only for Gram negatives, not for gram positives. You can use- QIAamp DNA minikit (Qiagen, Hilden, Germany).

Pawel Lisiecki

Please try with Bctozol Kit or DNAzol DNA extraction.

Alaa Kareem Niamah

I used boiling method for same bacteria .the method was bad DNA extract.the DNA extract from by boiling was counting. sediment

Please used DNA extraction kit

Gulnaz Bashir

Thank you all.I used GeneJet DNA purification kit (ThermoFisher ) for extraction with excellent results.