Which are the latest technologies a Clinical Microbiology laboratory should have for rapid diagnosis of infectious diseases in a hospital?

Need to upgrade our laboratory. To provide a timely diagnosis of various bacterial, viral, fungal and mycobacterial infections and do epidemiological typing of various nosocomial pathogens.

02 March 2018 137 4 View

I am getting a non specific band in PCR product using ITS3 and ITS4 at annealing temperatures of 56,57,58 and 59oC. Can anybody help?

I am getting a non specific band in PCR product using ITS3 and ITS4 at annealing temperatures of 56,57,58 and 59oC. Can anybody help?

08 September 2016 4,922 3 View

How do we remove amplicons from micropipettes?

My negative control is showing bands as in positive control. I repeated the experiment with new reagents and new primers. However the pipette used for adding template was used for gel loading also...

01 February 2016 5,052 4 View

Can anybody provide me a reference for speciation of filamentous fungi?

I want a reference on speciation of fungi especially zygomycetes.

11 December 2014 642 7 View

Why do the bands towards the electrodes move slowly in my electrophoresis unit?

09 October 2014 5,444 11 View

Which is the shortest and most reliable DNA extraction protocol for MTB from clinical samples?

Most of the protocols and even the commercial ones are time consuming.

07 August 2014 4,292 3 View

How good is the boiling method / sonication for DNA extraction from Enterococci?

I have tried both methods without any success.

06 July 2014 4,852 4 View

Is anybody growing H.pylori on culture and doing its antibiotic susceptibility. Can you share your experience?

I am in the process of collecting biopsies for the same.

03 April 2014 9,761 31 View

Which is the current best and easy PCR based method to speciate yeasts?

I am doing PCR-RFLP to identify yeasts. I want to do away with the steps of RFLP. I know of a multiplex PCR using 3 primers for identification.

03 April 2014 2,737 2 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View