I am getting a 1.8kb sized band in 1.8kb PCR encoding 16S-23S rRNA spacer region and their flanking parts with a reference H37 Rv strain but with none of the rapidly growing mycobacteria. What could be the reason ?
The primers is not suitable mycobacteria DNA?
You should compare the primers and mycobacteria genome sequence which probablely been searched from NCBI.
Or rapidly growing mycobacteria 16S-23S rRNA spacer region have mutated, You should design other primers.
Thanks Cheng
Lot of work has been done with such primers by other authors previously. can you suggest any other primer sequence to identify atypical mycobacteria.
Give me your reforence sequence.
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