Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Distortion problem in Abaqus explicit?

Dear all. I'm modeling the RC column with cyclic displacement by Abaqus-Explicit. The concrete model used Concrete Plastic Damage model. However I'm facing with a error about the excessive...

01 February 2021 7,726 1 View

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11 January 2021 3,284 3 View

Is there a generalized method to derive current-mode model of any DC-DC converters?

Hi all, As new DC-DC converters are being proposed, associated control method should be proposed and based on the small-model signal analysis of the DC-DC converters. Using average space state...

06 January 2021 7,247 3 View

How can we measure the surface relative content of deconvoluted Mn 2p3/2 XPS spectra?

Somewhere in papers, it is written as Mn 2p3/2 XPS spectra can be deconvoluted into four peaks at 640.83, 641.92, 642.97 and 644.56 eV assigned to Mn(II), Mn(III), Mn(IV), and the shakeup peak,...

24 December 2020 4,323 4 View

If we are going for co digestion of microalgae with wastewater but the sample is having low pH, What are the effective ways to increase the pH ?

wastewater with low pH

07 December 2020 2,716 3 View

How can we turn a short peptide having affinity for some compound into whole cell biosensor?

Greetings, I have a short stretch of 20 aa with strong binding affinity for a compound. I am trying to de some whole cell flourescence or visible biosensing technique based on this peptide. Can...

26 November 2020 8,322 3 View

How to intepret data with significant ANOVA but not significant Tukey test?

I have this set of data: Treatment 1: 30.5; 34.5; 24.4 Treatment 2: 24.8; 20.8; 16.8 Treatment 3: 19.1; 21.4; 21.0 Treatment 4: 22.3; 26.1; 27.1...

25 November 2020 920 4 View

Acesulfame Potassium and Acesulfame and Conversion?

Dear to whom it may concern, Based on ChemAxon, I calculated pKa values of Acesulfame as follows: 1. pKa (Strongest Acidic): 3.02 2. pKa (Strongest Basic): -6 Because I would like to convert...

25 November 2020 4,577 3 View

QUANTUM INFORMATION: Why a Toffoli gate can be used to obtain any permutation gate?

Hi, i am sudying some quantum computer science and I am really struggling to find an explanation to the question done above. Any kind of help would be wonderfull. Greets.

09 November 2020 4,657 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View