Does anyone have experience with plant meristem culture? What kind of plant growth regulator combination is best?
From what I read, there seem to be 2 methods. One using cytokinin (BAP, kinetin) combined with auxin (NAA, IBA) that reminiscing shoot cluster generation from leaves or callus. I presume that is also what being attempted here, turning the meristem tissue into callus and then to shoot cluster. Another method though uses cytokinin (BAP, kinetin) combined with gibberellin (GA3), which I presume is to maintain and elongate the meristem back into a single shoot tip instead of turning it into a shoot cluster.
What are your experiences with any of these methods? Which one worked for you, or do you have a different method?
The hormonal balance must be respected between cytokine, auxin and giberelic acid.5 mg/l of BAP+0.01mg/l of NAA+1mg/l of GA3 promote the regeneration of meristems in vitro
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