Ha Pham

5 Questions 3 Answers 0 Followers

Questions related from Ha Pham

How can I know my sample (cell medium) are too condense of cytokine before performing ELISA?

1. When performing ELISA, we have 2 different panels: standard and sample. In western blot, we do BSA assay at first to quantify protein concentration, so if the protein concentration is too much,...

11 October 2019 3,910 1 View

Can I do Immunohistochemistry (IHC) on mice tumor section with a chemokine like MIP-1a?

If I want to test a chemoattractant (it can recruit immune cells to tumor) such as MIP-1a, should I do IHC of tumor stained with MIP-1a staining? I want to test whether after treating with a drug,...

04 October 2019 3,392 4 View

How can I stimulate Jurkat E6.1 T cells?

As I know, Jurket E6.1 is naive T cell, so we need to stimulate them to become CD8 or CD4T cell. Does anyone know which stimulator I should used to differentiate Jurkat E6.1 cell to become...

03 October 2019 8,042 2 View

Human CD8 T cell recommendation?

Hi all, I need to work with CD8 Effector T cell and PD1+ CD8+ T cell. Does anyone know which cell line I can use? I have found out TALL-104 is human T cell but I don't know whether it expresses...

01 January 1970 2,685 2 View

Which stimulator used to up-regulate TIM3 and CD152(CTLA-4) immune checkpoint on T cell?

I am going to doing flow cytometry with splenocytes from mice. Does anyone know which stimulator people used to up-regulate CD153 and TIM3 so that I can see it clearly on flow cytometry? Thank...

01 January 1970 7,821 0 View