Ha Pham

6 Questions 3 Answers 0 Followers

Questions related from Ha Pham

How to use lung cancer xenograft mouse to study immune cell population?

I'm currently working on immune system changes in NSCLC. Does anyone know any paper related to this subject so I can have some references: 1. How many days after injection should I resect tumor...

12 December 2019 9,845 0 View

How can I know my sample (cell medium) are too condense of cytokine before performing ELISA?

1. When performing ELISA, we have 2 different panels: standard and sample. In western blot, we do BSA assay at first to quantify protein concentration, so if the protein concentration is too much,...

11 October 2019 3,986 1 View

Can I do Immunohistochemistry (IHC) on mice tumor section with a chemokine like MIP-1a?

If I want to test a chemoattractant (it can recruit immune cells to tumor) such as MIP-1a, should I do IHC of tumor stained with MIP-1a staining? I want to test whether after treating with a drug,...

04 October 2019 3,428 4 View

How can I stimulate Jurkat E6.1 T cells?

As I know, Jurket E6.1 is naive T cell, so we need to stimulate them to become CD8 or CD4T cell. Does anyone know which stimulator I should used to differentiate Jurkat E6.1 cell to become...

03 October 2019 8,071 2 View

Human CD8 T cell recommendation?

Hi all, I need to work with CD8 Effector T cell and PD1+ CD8+ T cell. Does anyone know which cell line I can use? I have found out TALL-104 is human T cell but I don't know whether it expresses...

01 January 1970 2,722 2 View

Which stimulator used to up-regulate TIM3 and CD152(CTLA-4) immune checkpoint on T cell?

I am going to doing flow cytometry with splenocytes from mice. Does anyone know which stimulator people used to up-regulate CD153 and TIM3 so that I can see it clearly on flow cytometry? Thank...

01 January 1970 7,849 0 View