Dear   Lili Liu

Pls. find the attached files and the references, good luck

Hoping this will be helpful to your project

Regards

Prof. Houda Kawas

http://www.nature.com/nprot/journal/v2/n11/full/nprot.2007.448.html

https://www.neb.com/products/e8033-anti-m13-piii-monoclonal-antibody

http://www.creativebiomart.net/description_10034_27.htm

https://www.abdesignlabs.com/store/index.php?dispatch=products.view&product_id=29804

https://www.bio-rad-antibodies.com/viral-m13-bacteriophage-antibody-e1-mca1858r.html

https://www.thermofisher.com/antibody/product/M13-Phage-coat-protein-Antibody-clone-E1-Monoclonal/MA1-12898

https://www.fitzgerald-fii.com/m13-fd-f1-filamentous-phages-antibody-hrp-61r-m101ahrp.html

http://www.gelifesciences.com/webapp/wcs/stores/servlet/catalog/en/GELifeSciences-fr/products/AlternativeProductStructure_17002/27942101

Article Open sandwich ELISA: A novel immunoassay based on the interc...

Also

http://www.abcam.com/m13-antibody-b62-fe2-hrp-ab50370.html

http://www.sigmaaldrich.com/catalog/product/sigma/ge27942101?lang=en®ion=SY

https://www.mybiosource.com/products.php?keywords=anti+M13+BACTERIOPHAGE+antibody

https://www.fishersci.com/shop/products/anti-m13-hrp-conjugate-monoclonal-ge-healthcare-1-each-hrp-conjugate/45001419

https://books.google.com/books?id=nFcrs_dGbxYC&pg=PA99&lpg=PA99&dq=HRP/Anti-M13+Monoclonal+antibody+for+phage+ELISA&source=bl&ots=3ZXvD4F8AW&sig=f-DdiUmSERtKx0rMTq-GlU-o7qc&hl=ar&sa=X&ved=0ahUKEwjh7uPKv-nSAhWiYJoKHXq0DVs4ChDoAQhUMAg#v=onepage&q=HRP%2FAnti-M13%20Monoclonal%20antibody%20for%20phage%20ELISA&f=false

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2505340/

http://www.creative-diagnostics.com/Anti-M13-MAb-HRP-Conjugated-130993-491.htm

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0126571

http://www.sciencedirect.com/science/article/pii/S2212553116000042

http://www.biocompare.com/pfu/110447/soids/2282/Antibodies/M13_bacteriophage

And

Hoping this will be helpful to your project

https://www.antibodyresource.com/search/Antibodies/3abbfb7d-786d-5966-9b03-e3771003e237/m13-phage-coat-protein

http://www.gelifesciences.com/webapp/wcs/stores/servlet/catalog/en/GELifeSciences-fr/products/AlternativeProductStructure_17002/27942101

http://www.gelifesciences.com/webapp/wcs/stores/servlet/productById/en/GELifeSciences-fr/27942001

http://www.sigmaaldrich.com/catalog/product/sigma/ge27942001?lang=en®ion=SY

http://www.biocompare.com/pfu/110447/soids/2282/Antibodies/M13_bacteriophage

http://www.opticsplanet.com/ge-anti-m13-monoclonal-antibody-27-9420-01.html

http://www.labplanet.com/ge-hrp-anti-m13-monoclnl-conjug-27-9421-01.html

http://www.fibercellsystems.com/applications/monoclonal-antibodies/

Hi Lili, did you find a suitable replacement? I have been trying to optimize the Abcam antibody but I found the 1/20 dilution ratio unrealistic for routine analysis considering only 250 ul is supplied per purchase.

http://www.abcam.com/m13-antibody-b62-fe2-hrp-ab50370.html

Yes I have the same problem. I thought the solution was the abcam antibodies, but after I purchased it, I noticed that I could do 1 ELISA assay with the 250µl tube. Id anyone has a solution, please share it 

We tested the M13/HRP ab from scbt (cat: sc-53004-HRP) recently. The signal at 1:1000 dilution can catch up the one from GE at 1:5000 dilution. You can try in your hand.

Thank you Lili, so you did ELISA with it? And they just give the concentration but I don't find the volume?

I replaced the Abcam with Sino Biological anti-M13 HRP-conjugated (cat: 11973-MM05-H) at dilution, also, of 1:1000. This seems to be a helpful replacement.

Thank you Mwila

Welcome. All the best!

Hi Mwila and Lili, at which concentration was the antibody? Cause I found 50, 100 and 200µg/ml, so I guess the 1/1000 dilution depends on the original concentration

Hi Larbi, sorry for the late reply. I was supplied with 100µg of the antibody at concentration of 0.83 µg/µl which is also my original concentration.

Good Morning, I asked a sample of this mAb to Santa Cruz Biotech and I compared it side by side with the mAb (without HRP) from GE and worked beautifully. I titrated the antibody and it is has to be used 5X more concentrate but still it was 1/5000 dilution from the stock (200 ug/mL).

The only issue is the extra step with the anti-mouse HRP (1/5000) but I am very happy that this is a good alternative. The company also has the HRPversion of this antibody but they didnt have samples of it, but I am going to buy it and I will post a graph making a comparation with the mAB:HRP from GE.

https://www.scbt.com/scbt/product/m13-major-coat-protein-antibody-rl-ph2

Thank you Martin. I've ordered the HRP version from Santa Cruz 1 month ago and I am stiil waiting for it

Let me know about it. Why is taking so long? Best luck!

Thank you, I will! They didn't produced it until this week, and it still has to pass the quality control

Hi Larbi, how is the anti-M13 HRP antibody from Santa Cruz? 

Hi, I have finally ordered the Sinobiological Antibody 11973-MM05T-H since the one from Santa Cruz didn't passe the quality control. I receieved it few days ago and I will share with you my experience when I do the ELISA. FYI, they recomend to use it at a concentration between 0.1 and 0.4µg/mL for ELISA, so it means that it can be deluted between 2000 and 8000 times. I think I am going to use it at 0.4 but I still haven't decided yet since Mwila used it at a concentration of 0.8

Hello Natalia, may i know the catalog number for the anti-M13-HRP from Santa Cruz that you use? Thank you.

Hi folks

had the same problem finding a replacement for the GE a-M13-HRP antibody and eventually ended up buying and testing the one from SinoBiological (cat# 11973-MM05T-H). Seeing as many of you where looking for a replacment too I thought I quickly share my results here.

Sino recommends using the antibody in a concentration range 0.1-0.4 ug/ml. I ordered 50 ug which came as a concentrated solution with 1.1 mg/ml. I'm mainly going to use this ab for phage ELISAs after panning so I titrated the ab using a set up that would resemble a polyclonal phage ELISA (10ug/ml antigen coating, 2-3x10^10 pfu per well, blocking with PBS+2%milkpowder). I ran the titration with a pool of binders that was known to bind quite strongly to the antigen.

Basically what I did was testing ab concentrations b/w 0.05-0.4ug/ml (corresponds to 1:2750 - 1:22000 with this lot) and saw that even 0.05 ug/ml was totally sufficient to get a good signal. When testing a pool of binders that are less mature you might need to increase the concentration a bit but I'd still say 0.1ug/ml of this ab is totally enough (1:11000).

Hope that helps.

Cheers

Has anyone tested the Origene Ab? BM5516HRP ?

Especially to Larbi Mokhtari and @Mwila Hilton Mwaba (whom somehow I can't tag): have you used the SinoBiological 11973-MM05T-H? Could you tell me at which concentration, and possibly also how many phages you put per well? Thank you in advance.

Iris Villani Sorry for the very late reply. I was using 1:1000 concentration though I suppose you could try 1:2000 to try and reduce usage. Did you succeed?

Hi, an alternative to anti-M13 HRP from GE, which is not available anymore, is the B62-FE2 HRP Conjugate: https://www.progen.com/anti-m13-fd-f1-filamentous-phages-mouse-monoclonal-b62-fe2-hrp-conjugate.html

Best wishes,

Michael

Sinobiological Antibody 11973-MM05T-H @1/4000x dilution is fine

Among all the one I tested Sinobiological Antibody 11973-MM05T-H is the one that works better at a dilution of 1:4000. I have not used the one suggested by Micheal Hust but it is more expensive than the one produced by Sinobiological.

Hi,

the antibody recommended by Michael Hust is also available in sample size https://www.progen.com/anti-m13-fd-f1-filamentous-phages-mouse-monoclonal-b62-fe2-hrp-conjugate-sample.html.

So, if you still search for an alternative to the GE antibody, maybe it is worth trying.

Caroline Odenwald thanks for the suggestion and I might rerequest a samples if you kindly can provide with one. The stock concentration is 100 ug/mL but the figure legend said " 1:1000, 10 ug of antibody per well". This do not make sense as the vial contain 25 ug of antibody and 1:1000 of the stock is 100 ng/mL (10 NG per well). Do you have districutors in Canada? Thank you and have a nice week