Iris Villani

2 Questions 5 Answers 0 Followers

Questions related from Iris Villani

(Western blot) Why would an antibody work on tissue lysates but not cell lysates?

I'm doing a denaturing western blot on tissue lysates and cell lysates. I have an antibody that seems to work on the former (shows a band only in tissues where it is known to be expressed), but...

10 January 2022 2,413 8 View

[Mass Spectrometry] fat in tissue lysates: shall I remove it or keep it (thinking of lipophylic proteins)?

Hello, I'm trying to analyze the proteome of some tissue by mass spectrometry. After lysing the samples and centrifuging them, sometimes there is quite a bit of fat floating on top of the...

14 July 2021 5,961 3 View