Fortunato Ferrara

6 Questions 20 Answers 0 Followers

Questions related from Fortunato Ferrara

Has anyone used recombinant proteins provided by mybiosource.com?

There is a recombinant protein of interest that they provide which I might be interested to use in phage selections.

13 February 2020 3,572 2 View

What is the best FACS out there?

I have been using FACAria II (upgraded later to III) for the last 9 years to sort from yeast, to bacteria to mammalian cells. Specially after the fluidic upgrade, I really cannot complain about...

28 November 2017 9,984 2 View

Which is the best mRNA purification kit?

I would like to have an updated answer to this question. I am looking for a easy but high yield kit (Miltenyi? Qiagen? Dynabeads?) Has anyone experience with the Miltenyi one? Thanks

01 November 2016 5,473 5 View

For a flow-cytometry experiment, what are the best "fluorescently labeled" anti-human-IgG and IgA antibodies?

To detect human IgG and IgA from serum samples in flow cytometry.

13 March 2014 8,173 2 View

Why my recombinant polyclonal antibodies don't recognize their target in WB?

We produce in vitro recombinant antibodies for different targets. When tested in ELISA -using folded target proteins - they all specifically recognize their target, but around 25-30% of them don't...

29 July 2013 1,535 4 View

Has anybody ever tried to use Baculovirus protein extract to pre-clear phage before a selection campaign?

One of the tests performed to check the polyspecificity of an antibody selected in vivo or in vitro is to test it in ELISA against BVP extract. My idea is to start already during the selection...

01 January 1970 4,152 2 View