Does HPLC grade reagents serve for UHPLC-MS quantification? Even filtered with 0.22 micron pore? Thanks in advance
The HPLC grade solvent is made to have less UV absorbance which is not relevant to the MS. HPLC is the same as UPLC except fir the latter one is done at a high pressure environment because the particle size of the stationarybphase is smaller . The anwer is no difference andbyou can use it
There are solvents specially designated "Chromasolv - tested for UHPLC", by Fluka, for example. Very expensive, but you can't go any lower for fine applications. And yes indeed, if you start filtering your HPLC solvents, or in any way try to improve them, you'll create more harm than good.
Use Riedel-de Haën, Burdick and Jackson, Honeywell any of these brand its same. Surely very costly.. but 100 % reliable.
we have used the HPLC grade solvents like acetonitrile/methanol/meluqie water for making mobile phase in UHPLC and LC-MS and there is no problem. the only thing observed is long life time in the case of LC-MS slowly eluent.
LC-MS solvents undergo several distinct tests to ensure they meet the stringent criteria required for sensitive LC-MS and LC-UV analyses. Some of the most important features are:
- Application-tested for LC-MS using the reserpine test
- Very low level of inorganic and metal ions for high sensitivity and spectral interpretation
- Free of particles and non-volatile compounds to maintain system integrity
- High UV-transmittance for UV-Diode Array applications
- Low gradient baseline even with your own optimized protocols
UHPLC solvents should be filtered to at least 0.1 um to work reliably in a UHPLCMS application. Also, HPLC solvents are not typically checked for MS background. Using an HPLC solvent in a UHPLC application is playing russian Roulette with your instrument, sample and columns.
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