Anthony Desbien

6 Questions 4 Answers 0 Followers

Questions related from Anthony Desbien

How do I reduce auto fluorescence when isolating cells from adipose tissue for flow cytometry?

Looking for tips regarding isolating svfs and lymphocytes. In particular how to reduce the autofluorescence from lipids. Any suggestions would be greatly appreciated.

04 May 2025 2,376 2 View

Is there a short tandem repeat (STR) database for mouse cell line authentification?

Hi, Have a mystery cell line of mouse origin that I need help identifying. I used the IDEXX service for STR analysis but the results were inconclusive. Seems like the database is forthcoming but...

30 April 2019 6,144 1 View

How would you distinguish granulocytic myeloid derived suppressor cells from a neutrophil?

The challenging part of this is that once a tumor has collapsed, presumably infiltrating neutrophils will "mop up" and look like granulocytic MDSC's i.e. ly6c mid, ly6g hi, cd11b+.

08 March 2016 3,275 3 View

What is a non-glycerol mounting media for immunofluorescence?

I would like to mount some tissue sections that contain a lipophilic dye that bleeds with glycerol-based mounting media. Do you have any favorites? Antifade reagents?

24 March 2015 5,673 4 View

Is hair a problem with NIR dylight 747 whole body imaging?

I need to image mice with a whole animal imaging platform and I'm wondering if it is necessary to remove hair with dylight747. I'll be image c57bl6.

11 September 2014 2,527 3 View

Can anyone help me with a CD8b depleting antibody?

I don't want to deplete CD8a dendritic cells but I want to remove CD8 memory cells. Anyone know of a good clone?

24 March 2014 8,537 3 View