I am working on B cell differentiation ,I want to know how self renewal can be experimentally assayed in these cells.
Dear Satyajeet,
The attached review article covers the answer to your question:
Wiley Interdiscip Rev Syst Biol Med. Author manuscript; available in PMC 2011 Nov 1.
Published in final edited form as:
Wiley Interdiscip Rev Syst Biol Med. 2010 Nov-Dec; 2(6): 640–653.
doi: 10.1002/wsbm.86
PMCID: PMC2950323
NIHMSID: NIHMS163761
Hematopoietic Stem Cell: Self-renewal versus Differentiation
Jun Seita* and Irving L. Weissman*
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The publisher's final edited version of this article is available at Wiley Interdiscip Rev Syst Biol Med
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Abstract
The mammalian blood system, containing more than ten distinct mature cell types, stands on one specific cell type, hematopoietic stem cell (HSC). Within the system, only HSC possess the ability of both multi-potency and self-renewal. Multi-potency is the ability to differentiate into all functional blood cells. Self-renewal is the ability to give rise to HSC itself without differentiation. Since mature blood cells are predominantly short lived, HSC continuously provide more differentiated progenitors while properly maintaining the HSC pool size properly throughout life by precisely balancing self-renewal and differentiation. Thus, understanding the mechanisms of self-renewal and differentiation of HSC has been a central issue. In this review, we focus on the hierarchical structure of the hematopoietic system, the current understanding of microenvironment and molecular cues regulating self-renewal and differentiation of adult HSC, and the currently emerging systems approaches to understand HSC biology.
Hoping this will be helpful,
Rafik
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