Proteomics
From soil we can isolate the bacetria/fungi by traditional serial dilution techniques. If anyone working on biodegaradtion than let me know about the screening parameters for microbes with respect...
03 April 2019 9,817 10 View
Methyl halide pesticide cell biology/molecular biology in insect
03 April 2018 1,163 5 View
basis of selection of mutation on enzyme active sites.
03 April 2018 524 3 View
Bioinformatics question
02 March 2018 8,043 10 View
Biochemical test and molecular tools of microorganisms??
02 March 2018 9,001 0 View
I want to know about the Fuzzy analysis in Biodegradation. Can anyone have Idea how to use fuzzy modeling for biodegradation of xenobiotics.????
31 December 2017 9,901 1 View
Protein isolation
04 May 2017 8,991 2 View
statistical error
02 March 2017 5,855 0 View
gene knockout
02 March 2017 5,975 0 View
Genome wide analysis in bacteria
09 October 2016 6,159 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
What is the role of oxidative stress in toxin-induced carcinogenesis? I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from...
02 August 2024 3,174 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...
30 July 2024 1,040 1 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View
i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....
26 July 2024 1,887 0 View
