What is the basis of selecting the bacteria/fungi from soil responsible for the biodegradation????

From soil we can isolate the bacetria/fungi by traditional serial dilution techniques. If anyone working on biodegaradtion than let me know about the screening parameters for microbes with respect...

03 April 2019 9,632 10 View

How methyl halide kill pest/insect and which types of pest it killed ? I want to know the exact mechanism in pest after methyl halide exposure????

Methyl halide pesticide cell biology/molecular biology in insect

03 April 2018 988 5 View

I want to do mutagenesis using insilico tools. What is the basis of mutant selection. How can I can prepare best mutation in enzyme active site?????

basis of selection of mutation on enzyme active sites.

03 April 2018 340 3 View

In Silico work how much fruitful in biological sciences??

Bioinformatics question

02 March 2018 7,812 10 View

Biochemical test of microorganisms have any importance as compare to recent molecular highthroughput tools...??

Biochemical test and molecular tools of microorganisms??

02 March 2018 8,707 0 View

I want to know about the Fuzzy analysis in Biodegradatiuon. Can anyone have Idea how to use fuzzy modeling for biodegradation of xenobiotics.????

I want to know about the Fuzzy analysis in Biodegradation. Can anyone have Idea how to use fuzzy modeling for biodegradation of xenobiotics.????

31 December 2017 9,714 1 View

How much error can be possible during experiment ? statistically 0.1% or 0.5% accepted ?

statistical error

02 March 2017 5,684 0 View

How we will ensure the isolated proteome contains all types of proteins during particular stress conditions.?

Proteomics

02 March 2017 9,174 0 View

Any one have idea how to knockout gene from bacteria??

gene knockout

02 March 2017 5,793 0 View

I want study the Pesticide degrading genes in bacteria through Genome wide identification Can Anyone guide for the same?

Genome wide analysis in bacteria

09 October 2016 5,992 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View