In field studies, ethephon used as spraying agent, just to enhance growth, fruit ripening and flowering etc...
Actually i want to use Ethephon and triacontanol (plant growth regulator) in plant tissue culture. Is Ethephon and triacontanol used in liquid culture or suspension culture? or semisolid culture?
IF YES
Then How much concentration we can used ?
Any protocol / method ?
Fraternale, D, Giamperi, L, Ricci, D and Rocchi, MBL. 2002. Micro-propagation of Bupleurum fruticosum L.: The effect of triacontanol.
Verma, A, Malik, CP, Sinsinwar, YK and Gupta, VK. 2009. Yield parameters responses in a spreading (cv. M-13) and semi-spreading (cv. Girnar-2) types of groundnut to six growth regulators.
Grzegorczyk, I, Bilichowski, I, Mikicuik-Olasik, E and Wysokinska, H. 2006. The effect of triacontanol on shoot multiplication and production of antioxidant compounds in shoot cultures of Salvia officinalis L.
Tantos, A, Meszaros, A, Farkas, T, Szalai, J and Horvath, G. 2001. Triacontanol-supported micropropagation of woody plants.
Tantos, A, Meszaros, A and Kissimon, J. 1999. The effect of triacontanol on micropropagation of balm, Melissa officinalis L.
I have never used. But I doubt if ethylene has any positive effect in tissue culture
Kourosh Vahdati ok thank you for response.
Yes ethylene have positive effect in plant tissue culture. In literature, which have seen in various plants for e.g. mung bean, Prunus avium etc....
Triacontanol increase the vegetative growth, chlorophyll content of plants, therefore, it is common to use it in the multiplication stage of PTC such as apples rootstock. Ethephon can be converted to ethylene in medium culture, but in prior studies, there is no significant effect on tissue culture.
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