I would like to ask to everyone expert worldwide, since cell culturing gives taste in your experiments, I mean commonly used to cultivate various cell lines intended for your research studies and selling cell lines to everyone. If you having bunch of FBS bottles in your stock with following descriptions ( 1. Heat inactivated FBS 2. Gamma Irradiated FBS 3. Dialyzed FBS 4. Certified and qualified FBS 5. FBS produced from USA, Australia and New Zealand. ) . What best to choose to avoid wastage any of 5 listed. What the effect of each variety listed. Should we choose only one for its cell lines we usually growing? Thank you.
Choice of the FBS depends partly on the cell lines. If you want to culture cells such as immune cells or smooth muscle cells you should inactivate complement and position 1. is appropriate. If you culture other cell types, this is not needed. Gamma irridiation is the most commonly used method to sterilize FBS - this in my opinion does not mean that other FBS products use another method. It appears, on the other hand, unlikely that you can buy not-sterilized FBS. Certified FBS is "better" than qualified FBS because additional testing has been performed. The certified may be needed if you use hormone-responsive cells. 5. describes the origin of the FBS, which may be important in a GMP operated facility. However, in this case, synthetic sera or platelet lysate is more appropriate.
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