Can we use 1X PBS or Diionized water as sheath fluid for Flow Cytometry?

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Sebastian J. Hofer

We are using autoclaved 1x PBS in ddH2O as sheath for a BD FACS. Should be fine, but depends on your experimental setup and technical demands

Himanshu Gogoi

It works fine with 1xPBS with 0.02 % sodium azide.

Muhammed Hamidur Rahaman

We regularly use deionised water as sheath fluid. However, we filter the water before using It.