Thadeo Trivilegio

5 Questions 1 Answers 0 Followers

Questions related from Thadeo Trivilegio

Best Specific Concentration For PBS ?

I have a big question in my mind while doing washing and processing tissue culture and PBMC's. I saw my coullege she used 10X PBS for washing but normally 1X PBS. Is there any effect on cells or...

25 August 2020 3,837 3 View

How to Utilized Variety of Fetal Bovine Serum?

I would like to ask to everyone expert worldwide, since cell culturing gives taste in your experiments, I mean commonly used to cultivate various cell lines intended for your research studies and...

18 July 2019 8,442 1 View

Can we use 1X PBS or Diionized water as sheath fluid for Flow Cytometry?

I am running out of sheath fluid , any help or recommendation from everyone who do flow cytometry? I am currently using BD FACS CANTO II. Thank a lot in advance.

29 September 2018 3,006 3 View

What the main cause failed bacterial plasmid transformation?

I am doing bacterial plasmid transformation on the newly purchased plasmid example FoxP3 merge with 10B competent cells. We used LB agar with ampicillin and inoculate the said mixture through it....

21 August 2017 6,058 3 View

Anyone one here who use Simon protein simple?

Anyone who use the simon protein simple. I need help how to manage regarding with antibody dilution, ratio and proportion for extracted proteins. i am facing problems how to adjust the...

17 March 2015 2,867 1 View